Different Forms of TFF3 in the Human Saliva: Heterodimerization with IgG Fc Binding Protein (FCGBP)
| Autor: | Werner Hoffmann, Till Houben, Harmut Schlüter, Sönke Harder, Hubert Kalbacher |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Proteomics Saliva Peptide lcsh:Chemistry TFF3 0302 clinical medicine Agglutinin lcsh:QH301-705.5 DMBT1 Spectroscopy chemistry.chemical_classification biology Chemistry trefoil factor General Medicine IgG Fc binding protein Computer Science Applications DNA-Binding Proteins Biochemistry 030220 oncology & carcinogenesis Trefoil Factor-3 Dimerization Glycan Catalysis Article Inorganic Chemistry 03 medical and health sciences mucin Polysaccharides Humans Protein Interaction Domains and Motifs Physical and Theoretical Chemistry Molecular Biology saliva Innate immune system Tumor Suppressor Proteins Organic Chemistry Mucin Calcium-Binding Proteins FCGBP Lectin Fast protein liquid chromatography Immunity Innate 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 biology.protein lectin Cell Adhesion Molecules |
| Zdroj: | International Journal of Molecular Sciences Volume 20 Issue 20 International Journal of Molecular Sciences, Vol 20, Iss 20, p 5000 (2019) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms20205000 |
| Popis: | The peptide TFF3 is a member of a family of secretory lectins, and is typically synthesized by mucous epithelia together with mucins. It is mainly released from intestinal goblet cells as a high-molecular mass heterodimer with IgG Fc binding protein (FCGBP). Herein, we investigated human saliva by fast protein liquid chromatography (FPLC) and proteomics and identified high- and low-molecular-mass forms of TFF3. Whereas the high-molecular-mass forms represent a heterodimer with FCGBP, the low-molecular-mass forms represent homodimeric TFF3 forms. Proteomic analysis also revealed a C-terminally truncated form of TFF3. We hypothesize that salivary TFF3-FCGBP might play a role in the innate immune defense of the oral cavity and that TFF3 might also bind to microbial glycans. The known interaction of TFF3 with the agglutinin DMBT-1, a typical constituent of human saliva, further supports this protective role. |
| Databáze: | OpenAIRE |
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