The potential of flow cytometric analysis for the characterization of hybridoma cells in suspension cultures
| Autor: | Jan W. Oberink, Tiny A. M. van der Velden-de Groot, José M. Coco-Martin, E. Coen Beuvery |
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| Rok vydání: | 1992 |
| Předmět: |
Cytoplasm
medicine.drug_class Cell Survival Clinical Biochemistry Molecular Sequence Data Biomedical Engineering Bioengineering Monoclonal antibody Epitope Flow cytometry Antigen Antibody Specificity medicine Amino Acid Sequence Hybridomas medicine.diagnostic_test biology Cell Cycle Cell Membrane Antibodies Monoclonal Cell Biology Cell cycle Flow Cytometry Molecular biology Kinetics Cell culture Evaluation Studies as Topic Immunoglobulin G biology.protein Antibody Cell Division Biotechnology |
| Zdroj: | Cytotechnology. 8(1) |
| ISSN: | 0920-9069 |
| Popis: | Flow cytometric (FC) analysis was applied to determine changes at cellular level during the cultivation of hybridoma cell line MN12 in a suspension batch culture. The relative cell size, cytoplasmic and membrane IgG content and the viability were monitored. Besides, the specificity of the cytoplasmic and membrane IgG was ascertained by means of a synthetic peptide containing the antigenic epitope recognized by the antibody. Cell size was found to increase during the exponential growth phase. The viability as determined by FC follows a similar pattern with the viability data obtained by the conventional trypan blue exclusion test. The relative cytoplasmic and membrane IgG contents were high during the exponential growth and low during stationary phase. Measurement of cell cycle distribution and the antibody content in the culture fluid, indicated that the major part of the cytoplasmic IgG is secreted by cells in the G1-phase. It is concluded that flow cytometry is a useful tool to characterize hybridoma cell lines in a suspension batch culture. |
| Databáze: | OpenAIRE |
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