Characterization of epithelial cell cultures derived from human tracheal glands
| Autor: | Gregory W. Taylor, Richard L. Shoemaker, Dharam P. Chopra, Patricia A. Mathieu |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Peanut agglutinin
Hydrocortisone Clinical Biochemistry Molecular Sequence Data Epithelium Ion Channels Membrane Potentials chemistry.chemical_compound Exocrine Glands Epidermal growth factor Culture Techniques medicine Serine Humans Insulin Secretion Hyaluronic Acid Bovine Pituitary Extract Cells Cultured Glucosamine Forskolin biology Epidermal Growth Factor Sulfates Tissue Extracts Mucins Epithelial Cells Cell Biology General Medicine Molecular biology Mucus Trachea Kinetics Microscopy Electron medicine.anatomical_structure chemistry Biochemistry Carbohydrate Sequence Cell culture Pituitary Gland biology.protein Cell Division Developmental Biology |
| Zdroj: | In vitro cellulardevelopmental biology : journal of the Tissue Culture Association. 27(1) |
| ISSN: | 0883-8364 |
| Popis: | Cultures of normal human tracheal gland epithelial cells that exhibit functional differentiation have been propagated in serum-free medium supplemented with insulin (5 micrograms/ml), epidermal growth factor (10 ng/ml), hydrocortisone (0.5 micrograms/ml), and bovine pituitary extract (25 micrograms/ml). The cells retain many characteristics of epithelial cells including microvilli on cell surfaces, desmosomes between cells, and tonofilaments in the cytoplasm. In addition, they exhibit keratin-positive titers and react positively with Peanut agglutinin, which is specific for the disaccharide beta-D-galactose-(1----3)N-acetyl D-galactosamine, a major component of mucin glycoprotein. The cells also exhibit normal Cl- channel activity which was enhanced by the cAMP agonist Forskolin. The major component of the cellular secretion was hyaluronic acid; approximately 10% of the void volume material was resistant to hyaluronidase and may contain material similar to mucin glycoprotein. Some of the cell cultures have been maintained in serum-free conditions for 6 to 7 passages. This model will be important to study regulation of ion-channel activities and mucous glycoprotein secretion and to compare such regulations with the tracheal mucosal epithelial cells already established. |
| Databáze: | OpenAIRE |
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