Equine herpesvirus type 1: Detection of viral DNA sequences in aborted fetuses with the polymerase chain reaction
| Autor: | J. Flensburg, B. Klingeborn, Sándor Belák, A. Ballagi-Pordány |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Base pair
Placenta Equine herpesvirus 1 Polymerase Chain Reaction Microbiology Virus law.invention chemistry.chemical_compound Fetus Pregnancy law Animals Horses Herpesviridae Polymerase chain reaction Electrophoresis Agar Gel General Veterinary biology Hybridization probe Nucleic Acid Hybridization Herpesviridae Infections General Medicine Abortion Veterinary biology.organism_classification Virology Molecular biology chemistry DNA Viral Female Horse Diseases Oligonucleotide Probes Oligomer restriction Molecular probe DNA Herpesvirus 1 Equid |
| Zdroj: | Veterinary Microbiology. 22:373-381 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/0378-1135(90)90024-p |
| Popis: | Primers and probes were selected from the gene encoding glycoprotein 13 (gp13) of equine herpesvirus 1 (EHV-1). The polymerase chain reaction (PCR) was run on infected and noninfected cultured cells and on 63 specimens from 29 aborted equine fetuses. The results were evaluated by electrophoresis and dot-blot hybridization using an oligonucleotide probe labeled with biotin. In the infected samples electrophoresis showed a PCR product of about 280 base pairs. The dot-blot hybridization confirmed that this product contained EHV-1 DNA sequences. PCR took 4 h and hybridization another 14 h; the results were thus achieved within 24 h and were highly specific for EHV-1. Close concordance was found between the results of PCR and virus isolation. |
| Databáze: | OpenAIRE |
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