Longitudinal effects of developmental bisphenol A, variable diet, and physical activity on age-related methylation in blood
| Autor: | Joseph Kochmanski, Elizabeth H. Marchlewicz, Dana C. Dolinoy |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Bisphenol A bisphenol A Health Toxicology and Mutagenesis physical activity Biology 03 medical and health sciences chemistry.chemical_compound Internal medicine Genetics medicine Epigenetics Molecular Biology Gene Genetics (clinical) 2. Zero hunger DNA methylation epigenetics aging Methylation Epigenome 3. Good health high-fat diet 030104 developmental biology Endocrinology chemistry Genomic imprinting Estrogen receptor alpha Research Article |
| Zdroj: | Environmental Epigenetics |
| ISSN: | 2058-5888 |
| Popis: | Research indicates that environmental factors can alter DNA methylation, but the specific effects of environmental exposures on epigenetic aging remain unclear. Here, using a mouse model of human-relevant exposures, we tested the hypothesis that early-life exposure to bisphenol A (BPA), variable diet, and/or changes in physical activity would modify rates of age-related methylation at several target regions, as measured from longitudinal blood samples (2, 4, and 10 months old). DNA methylation was quantified at two repetitive elements (LINE-1, IAP), two imprinted genes (Igf2, H19), and one non-imprinted gene (Esr1) in isogenic mice developmentally exposed to Control, Control + BPA (50 µg/kg diet), Western high-fat diet (WHFD), or Western + BPA diets. In blood samples, Esr1 DNA methylation increased significantly with age, but no other investigated loci showed significant age-related methylation. LINE-1 and IAP both showed significant negative environmental deflection by WHFD exposure (P |
| Databáze: | OpenAIRE |
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