Identification of phenolic markers for saffron authenticity and origin: An untargeted metabolomics approach
| Autor: | Gabriele Rocchetti, Silvia Ghisoni, Luigi Lucini, Marcelino De Los Mozos Pascual, Biancamaria Senizza, J. A. Fernández, Marco Trevisan, Matteo Busconi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Hydroxybenzoic acid
Spectrometry Mass Electrospray Ionization 030309 nutrition & dietetics Food integrity ved/biology.organism_classification_rank.species Crocus sativus Food metabolomics Multivariate statistics Polyphenols Food Contamination Flowers Biology Flavones Anthocyanins 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Metabolomics Flavonols Phenols Settore AGR/13 - CHIMICA AGRARIA Food science Spices Chromatography High Pressure Liquid chemistry.chemical_classification Flavonoids 0303 health sciences ved/biology Discriminant Analysis 04 agricultural and veterinary sciences Crocus 040401 food science chemistry Polyphenol Protocatechuic aldehyde Identification (biology) Biomarkers Food Analysis Food Science |
| Popis: | Saffron is a high-quality and expensive spice being widely subjected to adulteration. An UHPLC-ESI/QTOF-MS metabolomic-based approach was therefore used to investigate the discrimination potential between adulterated (added with different percentage of other parts of the flower) and authentic saffron, as well as to trace its geographical origin. Both unsupervised (hierarchical clustering) and supervised OPLS-DA multivariate statistics allowed discriminating authentic saffron from styles added of other floral components, as well as PDO (Protected Designation of Origin) vs non PDO saffron samples according to their chemical fingerprints. The proposed markers were then validated through ROC curves. Anthocyanins and glycosidic flavonols were the best markers of the styles' adulteration. However, other flavonoids (mainly free flavonols and flavones), together with protocatechuic aldehyde and isomeric forms of hydroxybenzoic acid, were also validated as markers for the discrimination of PDO vs non PDO saffron samples. This work outlines the potential of untargeted metabolomics based on UHPLC-ESI/QTOF mass spectrometry for saffron authenticity and traceability. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|