Carbon nanopowder acts as a Trojan-horse for benzo(α)pyrene in Danio rerio embryos
Autor: | Luca Bini, Andrea Binelli, Claudia Landi, C. Della Torre, Daniela Maggioni, Stefano Magni, L. Del Giacco, Alessandro Armini, Anna Ghilardi, Miriam Ascagni, Laura Prosperi, L. Madaschi, Marco Parolini, C. A. M. La Porta, Nadia Santo |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Materials science Danio Biomedical Engineering chemistry.chemical_element Fossil fuel combustion 010501 environmental sciences Toxicology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound proteomics Benzo(a)pyrene Animals pollution 0105 earth and related environmental sciences Pollutant biology Embryo Contamination biology.organism_classification zebrafish Carbon Bioavailability 030104 developmental biology chemistry microscopy Nanoparticles Environmental chemistry Pyrene Environmental Pollutants |
Popis: | Carbon-based nanoparticles (CBNs) are largely distributed worldwide due to fossil fuel combustion and their presence in many consumer products. In addition to their proven toxicological effects in several biological models, attention in recent years has focussed on the role played by CBNs as Trojan-horse carriers for adsorbed environmental pollutants. This role has not been conclusively determined to date because CBNs can decrease the bioavailability of contaminants or represent an additional source of intake. Herein, we evaluated the intake, transport and distribution of one of the carbon-based powders, the so-called carbon nanopowder (CNPW), and benzo(α)pyrene, when administered alone and in co-exposure to Danio rerio embryos. Data obtained by means of advanced microscopic techniques illustrated that the “particle-specific” effect induced a modification in the accumulation of benzo(α)pyrene, which is forced to follow the distribution of the physical pollutant instead of its natural bioaccumulation. The combined results from functional proteomics and gene transcription analysis highlighted the different biochemical pathways involved in the action of the two different contaminants administered alone and when bound together. In particular, we observed a clear change in several proteins involved in the homeostatic response to hypoxia only after exposure to the CNPW or co-exposure to the mixture, whereas exposure to benzo(α)pyrene alone mainly modified structural proteins. The entire dataset suggested a Trojan-horse mechanism involved in the biological impacts on Danio rerio embryos especially due to different bioaccumulation pathways and cellular targets. |
Databáze: | OpenAIRE |
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