The Gut Microbiome Derived From Anorexia Nervosa Patients Impairs Weight Gain and Behavioral Performance in Female Mice
| Autor: | Xue Ting Zhang, Yasunari Asano, Natsuru Watanabe, Kazufumi Yoshihara, Makoto Yamashita, Shu Takakura, Yasuhiro Koga, Nobuyuki Sudo, Noriyuki Miyata, Tae Kimura-Todani, Katsunaka Mikami, Tomokazu Hata |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult medicine.medical_specialty Anorexia Nervosa Anorexia nervosa Weight Gain 03 medical and health sciences Mice Young Adult 0302 clinical medicine Endocrinology Internal medicine medicine Animals Germ-Free Life Humans Young adult Feces Mice Inbred BALB C Behavior Animal business.industry Fecal Microbiota Transplantation medicine.disease Gut microbiome Gastrointestinal Microbiome 030104 developmental biology Compulsive behavior Female Serotonin medicine.symptom business Weight gain Dysbiosis 030217 neurology & neurosurgery |
| Zdroj: | Endocrinology. 160(10) |
| ISSN: | 1945-7170 |
| Popis: | Anorexia nervosa (AN) results in gut dysbiosis, but whether the dysbiosis contributes to AN-specific pathologies such as poor weight gain and neuropsychiatric abnormalities remains unclear. To address this, germ-free mice were reconstituted with the microbiota of four patients with restricting-type AN (gAN mice) and four healthy control individuals (gHC mice). The effects of gut microbes on weight gain and behavioral characteristics were examined. Fecal microbial profiles in recipient gnotobiotic mice were clustered with those of the human donors. Compared with gHC mice, gAN mice showed a decrease in body weight gain, concomitant with reduced food intake. Food efficiency ratio (body weight gain/food intake) was also significantly lower in gAN mice than in gHC mice, suggesting that decreased appetite as well as the capacity to convert ingested food to unit of body substance may contribute to poor weight gain. Both anxiety-related behavior measured by open-field tests and compulsive behavior measured by a marble-burying test were increased only in gAN mice but not in gHC mice. Serotonin levels in the brain stem of gAN mice were lower than those in the brain stem of gHC mice. Moreover, the genus Bacteroides showed the highest correlation with the number of buried marbles among all genera identified. Administration of Bacteroides vulgatus reversed compulsive behavior but failed to exert any substantial effect on body weight. Collectively, these results indicate that AN-specific dysbiosis may contribute to both poor weight gain and mental disorders in patients with AN. |
| Databáze: | OpenAIRE |
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