TLC densitometric method for screening of lycopsamine in comfrey root (Symphytum officinale L.) extracts using retrorsine as a reference compound
| Autor: | Damjan Janeš, Samo Kreft |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Pharmaceutical Science
Comfrey lycopsamine Plant Roots retrorsine COMFREY ROOT chemistry.chemical_compound Symphytum officinale Severe toxicity Pharmaceutical industry Pyrrolizidine Alkaloids Pharmacology Chromatography biology Plant Extracts tlc General Medicine Repeatability Reference Standards biology.organism_classification Thin-layer chromatography Lycopsamine chemistry Pyrrolizidine Chromatography Thin Layer HD9665-9675 Densitometry |
| Zdroj: | Acta Pharmaceutica, Vol 64, Iss 4, Pp 503-508 (2014) |
| ISSN: | 1846-9558 |
| DOI: | 10.2478/acph-2014-0031 |
| Popis: | Due to severe toxicity of pyrrolizidine alkaloids, their quantification in medicinal products is very important. The idea of this research was to use retrorsine as a surrogate reference compound instead of lycopsamine reference or lycopsamine isolated from comfrey. A method for the analysis of lycopsamine in extracts of comfrey roots was developed and validated, employing thin layer chromatography, derivatisation with Dann-Mattocks reagent followed by densitometric analysis. The new method showed linearity within 0.70 to 7.0 μg of lycopsamine per application of 10 μL of a solution. It has also been proven to be specific and precise (repeatability RSD 2-4 % within the plate). The method was successfully employed for quantification of lycopsamine in comfrey root and comfrey root medicinal products such as ointments. |
| Databáze: | OpenAIRE |
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