Calcium-Independent Exo-endocytosis Coupling at Small Central Synapses
| Autor: | Dietmar Schmitz, Christian Rosenmund, Melissa A. Herman, Marta Orlando |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Synaptic Transmission Synapse 0302 clinical medicine metabolism [Calcium] lcsh:QH301-705.5 Mice Knockout Neurons Chemistry metabolism [Clathrin] Brain food and beverages physiology [Neurons] Endocytosis Synaptic vesicle exocytosis physiology [Nerve Tissue Proteins] Female Synaptic Vesicles Function and Dysfunction of the Nervous System Vesicle fusion metabolism [Actins] Presynaptic Terminals Nerve Tissue Proteins physiology [Presynaptic Terminals] macromolecular substances Neurotransmission Exocytosis General Biochemistry Genetics and Molecular Biology 03 medical and health sciences physiology [Brain] physiology [Synaptic Vesicles] Animals ddc:610 Active zone Actin cytology [Brain] fungi Actins Clathrin Mice Inbred C57BL 030104 developmental biology lcsh:Biology (General) cytology [Neurons] Synapses Biophysics Calcium physiology [Synapses] 030217 neurology & neurosurgery |
| Zdroj: | Cell Reports, Vol 29, Iss 12, Pp 3767-3774.e3 (2019) Cell reports 29(12), 3767-3774.e3 (2019). doi:10.1016/j.celrep.2019.11.060 |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2019.11.060 |
| Popis: | Summary: At presynaptic terminals, neurotransmitters are released by synaptic vesicle exocytosis at the active zone. In order to maintain efficient neurotransmission and proper synaptic structure, sites of vesicle fusion must be cleared rapidly by endocytosis. Therefore, the coupling of exo- and endocytosis is crucial. Despite many years of research, the exact molecular and biophysical requirements for the coupling of exo- and endocytosis remain unclear. We investigate whether endocytosis can be triggered in a calcium-independent fashion by evoking calcium-independent exocytosis using a hypertonic sucrose solution. We demonstrate that endocytosis can be triggered, in the absence of calcium influx, in a clathrin-independent manner that relies on actin polymerization. Our findings point to a central role of membrane tension dependent on actin for efficient coupling of exo- and endocytosis. : As Ca2+ is necessary for action-potential-evoked synaptic vesicle fusion, the role of Ca2+ in triggering endocytosis is difficult to discern. Orlando et al. find that Ca2+-independent exocytosis triggered by hyperosmotic shock results in the formation of endocytic pits in hippocampal synapses, which depend on actin polymerization. Keywords: endocytosis, calcium, presynaptic terminal, synapse, synaptic transmission, ultrastructure, exo-endocytosis coupling |
| Databáze: | OpenAIRE |
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