Characterisation of plant tocopherol cyclases and their overexpression in transgenic Brassica napus seeds
| Autor: | Jens Gruber, Margrit Frentzen, Dagmar Weier, Wilfried Lühs, Mirela Raclaru, Thomas Schüßeler, Wolfgang Friedt, Karim Zarhloul, Rajeev Kumar, Radin Sadre, Dieter Enders |
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| Rok vydání: | 2004 |
| Předmět: |
Rapeseed
Transgene medicine.medical_treatment Plastochromanol-8 Biophysics Brassica Arabidopsis Gene Expression Tocopherols medicine.disease_cause Genes Plant Biochemistry Zea mays Substrate Specificity Structural Biology Genetics medicine Vitamin E Tocopherol 2 3-Dimethyl-5-phytyl-1 4-benzoquinol Molecular Biology Escherichia coli Intramolecular Transferases biology Molecular Structure Tocopherol cyclase Brassica napus Temperature food and beverages Cell Biology Hydrogen-Ion Concentration biology.organism_classification Plants Genetically Modified Transgenic rapeseed Recombinant Proteins Genetically modified organism Seeds |
| Zdroj: | FEBS letters. 579(6) |
| ISSN: | 0014-5793 |
| Popis: | Tocopherols, collectively known as vitamin E, are only synthesised in photosynthetic organisms. Tocopherol cyclase (TC) catalyses the formation of the chromanol headgroup of the various tocopherol isoforms. TCs from Arabidopsis and maize (Zea mays) were expressed in Escherichia coli and purified. Analysis of the enzymatic properties revealed similarities but also differences between the two enzymes. Overexpression of chimeric TC gene constructs in developing seeds of transgenic rapeseed plants enhanced and modified the relative abundance of individual tocochromanol species in the seed oil, indicating a regulatory function of the enzyme in prenyllipid metabolism. |
| Databáze: | OpenAIRE |
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