Consequences of the exposure to bisphenol A in cell membrane models at the molecular level and hamster ovary cells viability
| Autor: | Osvaldo N. Oliveira, Cibely S. Martin, Dalita G. S. M. Cavalcante, Mateus D. Maximino, Aldo Eloizo Job, Carla Y. Silva, Priscila Alessio |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP) |
| Rok vydání: | 2021 |
| Předmět: |
Cell viability
endocrine system Bisphenol A Spectrophotometry Infrared AGENTE TÓXICO Hamster 02 engineering and technology 01 natural sciences Cell membrane chemistry.chemical_compound Colloid and Surface Chemistry Phenols Cricetinae 0103 physical sciences Monolayer Cell membrane models medicine Animals Humans Vesicles Viability assay Benzhydryl Compounds Physical and Theoretical Chemistry Endocrine disruptors Langmuir monolayers Aged 010304 chemical physics urogenital system Chemistry Vesicle Cell Membrane technology industry and agriculture Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology Membrane medicine.anatomical_structure Endocrine disruptor Biophysics lipids (amino acids peptides and proteins) 0210 nano-technology hormones hormone substitutes and hormone antagonists Biotechnology |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2021-06-25T10:28:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-07-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The inadequate disposal and the difficulty in its removal from water treatment systems have made the endocrine disruptor bisphenol A (BPA) a significant hazard for humans and animals. The molecular-level mechanisms of BPA action are not known in detail, which calls for systematic investigations using cell membrane models. This paper shows that BPA affects Langmuir monolayers and giant unilamellar vesicles (GUVs) of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) used as membrane models, in a concentration-dependent manner and with effects that depend on BPA aggregation. BPA increases DPPC monolayer fluidity in surface pressure isotherms upon interacting with the headgroups through hydrogen bonding, according to polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). In DPPC GUVs, BPA induced wrinkling and distortion in the spherical shape of the vesicles, but this was only observed for fresh solutions where it is not aggregated. BPA also decreased the viability of hamster ovary cells (CHO) in in vitro experiments. In contrast, aged, aggregated BPA solutions did not affect the GUVs and even increased CHO viability. These results may be rationalized in terms of size-dependent effects of BPA, which may be relevant for its endocrine-disrupting effects. São Paulo State University (UNESP) School of Technology and Applied Sciences São Carlos Institute of Physics University of São Paulo, CP 369 São Paulo State University (UNESP) School of Technology and Applied Sciences CNPq: 304836/2018-4 CNPq: 422163/2018-0 |
| Databáze: | OpenAIRE |
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