Full-term development of mouse eggs transplanted with male pronuclei derived from round spermatids: The effect of synchronization between male and female pronucleus on embryonic development

Autor:	Zi-Li Lei, Da-Yuan Chen, Yue-Liang Zheng, Zi-Yu Zhu, Man-Xi Jiang, Qing-Yuan Sun, Ying-Chun Ouyang
Rok vydání:	2005
Předmět:	Male Female pronucleus Spermatid Pronucleus Embryogenesis Fertilized oocyte Embryo Cell Biology Anatomy Biology Embryo Mammalian Spermatids Andrology Mice Fetus medicine.anatomical_structure embryonic structures Genetics medicine Animals Female Early cleavage Ovum Developmental Biology Full Term
Zdroj:	Molecular Reproduction and Development. 71:439-443
ISSN:	1040-452X
DOI:	10.1002/mrd.20299
Popis:	Pronucleus transplanted mice have been produced, but their donor male pronuclei were derived from mature sperm and were completely synchronous with female pronuclei because both male and female pronuclei came from the same fertilized oocyte. The present study firstly produced male pronuclei by introducing round spermatids into enucleated mouse oocytes, then transferred the male pronuclei into mouse oocytes at three activation stages and finally compared the effect of three kinds of oocytes on the development of reconstructed embryos. Our results indicate that, in enucleated oocytes, mouse round spermatid nuclei can transform to male pronuclei in a higher proportion, and the synchronization between male and female pronucleus does not significantly influence the early cleavage but the later and full-term development of reconstructed embryos.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd3acbf27a24126605617f3803eb9732 https://doi.org/10.1002/mrd.20299 Zobrazit plný text záznamu Plný text