Differences between antioxidant defense parameters and specific trace element concentrations in healthy, benign, and malignant brain tissues

Autor: Slavica Borković-Mitić, Aleksandar Stojsavljević, Slađan Z. Pavlović, Bojan M. Mitić, Siniša Matić, Ljiljana Vujotić, Dragan Manojlović
Rok vydání: 2021
Předmět:
0301 basic medicine
Male
Antioxidant
Physiology
medicine.medical_treatment
Glutathione reductase
medicine.disease_cause
Antioxidants
chemistry.chemical_compound
0302 clinical medicine
Glutathione Transferase
chemistry.chemical_classification
Multidisciplinary
biology
Molecular medicine
Chemistry
Brain Neoplasms
Glutathione peroxidase
Brain
Middle Aged
Catalase
Glutathione
Chemical biology
Enzymes
Zinc
Glutathione Reductase
language
Acetylcholinesterase
Medicine
Female
Adult
medicine.medical_specialty
Aché
Science
Gene Expression Regulation
Enzymologic
Article
Superoxide dismutase
03 medical and health sciences
Selenium
Internal medicine
medicine
Humans
Glutathione Peroxidase
Manganese
Superoxide Dismutase
language.human_language
Trace Elements
030104 developmental biology
Endocrinology
Case-Control Studies
biology.protein
030217 neurology & neurosurgery
Oxidative stress
Copper
Zdroj: Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
Scientifc Reports
ISSN: 2045-2322
Popis: There are only a few reports examining the impact of oxidative stress in patients with benign and malignant brain tumors. In this study we investigated whether there are changes in antioxidant system (AOS) parameters and key trace elements between control, benign and malignant brain tissues. The study also aimed to examine correlations between the analyzed parameters. The study enrolled both types of brain tumors, benign tumors (BT) and malignant tumors (MT). The results were compared with control tissue (CT) without tumor infiltration collected from patients with BT. The following antioxidant parameters were determined: activities of total, manganese-containing, and copper/zinc-containing superoxide dismutase (TotSOD, MnSOD and CuZnSOD), activities of catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase and acetylcholine esterase (AChE), the concentrations of glutathione and sulfhydryl groups and of manganese (Mn), copper (Cu), zinc (Zn), and selenium (Se). BT and MT had altered activities/levels of multiple AOS parameters as compared to CT, indicating that tumor cells had an altered cell metabolism and changes in AOS represent adaptive response to increased oxidative stress. Low MnSOD and AChE and high GST activities were significant for distinguishing between MT and CT. Malignant tissue was also characterized by lower Mn and Cu concentrations relative to CT and BT. Principal Component Analysis clearly discriminated BT from CT and MT (PC1, 66.97%), while PC2 clearly discriminated CT from BT and MT (33.03%). Most correlative relationships were associated with Se in the BT group and Cu in the MT group. The results of this study reveal differences between the AOS parameters and the essential trace elements between the analyzed groups. The observed dysregulations show that oxidative stress could have an important role in disrupting brain homeostasis and its presence in the pathogenesis of benign and malignant brain tumors.
Databáze: OpenAIRE
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