Glucose metabolism by preimplantation pig embryos
| Autor: | J. L. Wiebold, M. R. Flood |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Embryology
medicine.medical_specialty Swine Pentose phosphate pathway Biology Carbohydrate metabolism Morula Pentose Phosphate Pathway Endocrinology Culture Techniques Internal medicine medicine Animals Blastocyst reproductive and urinary physiology Ovum Estrous cycle Obstetrics and Gynecology Embryo Embryo culture Cell Biology Metabolism Glucose medicine.anatomical_structure Reproductive Medicine embryonic structures Energy source |
| Zdroj: | Reproduction. 84:7-12 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/jrf.0.0840007 |
| Popis: | Pig embryos were collected, 2-7 days after oestrus, in modified BMOC-2 containing glucose as the only energy source. Embryos were incubated individually in medium containing [5-(3)H]-, [1-(14)C]- or [6-(14)C]glucose. Total glucose metabolism, as measured by [5-(3)H]glucose use, increased steadily from the 1-cell to the 8-cell stage. Total glucose use increased (P less than 0.05) at the compacted morula stage and was highest (P less than 0.05) at the blastocyst stage. Production of 14CO2 from embryos metabolizing [1-(14)C]glucose increased steadily from the unfertilized ovum to the 8-cell stage. Metabolism of [1-(14)C]glucose increased at the compacted morula stage (P less than 0.05) and continued to increase (P less than 0.05) to the blastocyst stage. Metabolism of [6-(14)C]glucose increased steadily from the unfertilized ovum to the compacted morula stage. Metabolism of [6-(14)C]glucose was highest (P less than 0.05) for the blastocyst stage. Percentage pentose phosphate pathway activity of total glucose metabolism before the 4-cell stage was higher (greater than 5%) than that of 8-cell to blastocyst stage embryos (approximately 1%). When embryo metabolism was determined on a per cell basis for each isotope, the compacted morulae stage (16 cells) had a higher total glucose metabolism than all other embryo stages (P less than 0.05), while early blastocyst (32 cells) and blastocyst (64 cells) stage embryos metabolized more [5-(3)H]glucose than all stages except compacted morulae (P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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