Development and feasibility of quantitative dynamic cardiac imaging for mice using μSPECT
| Autor: | Daniel J. Rader, Lindsay C. Johnson, Stephen C. Moore, Marie A Guerraty, Scott D. Metzler, Eric Blankemeyer |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Technetium Tc 99m Sestamibi
medicine.medical_specialty Quantitative imaging Blood volume 030204 cardiovascular system & hematology Article 030218 nuclear medicine & medical imaging Microsphere 03 medical and health sciences Mice 0302 clinical medicine Internal medicine Coronary Circulation medicine Animals Radiology Nuclear Medicine and imaging Cardiac imaging Tomography Emission-Computed Single-Photon business.industry Blood flow Coronary Vessels Red blood cell Cardiac Imaging Techniques medicine.anatomical_structure Microvessels Cardiology Arterial blood Feasibility Studies Radiopharmaceuticals Cardiology and Cardiovascular Medicine business Perfusion |
| Zdroj: | J Nucl Cardiol |
| ISSN: | 1532-6551 |
| Popis: | BACKGROUND: Despite growing interest in Coronary Microvascular Disease (CMVD), there is a dearth of mechanistic understanding. Mouse models offer opportunities to understand molecular processes in CMVD. We have sought to develop quantitative mouse imaging to assess coronary microvascular function. METHODS: We used (99m)Tc-Sestamibi to measure myocardial blood flow in mice with MILabs U-SPECT(+) system. We determined recovery and crosstalk coefficients, the influx rate constant from blood to myocardium (K1), and, using microsphere perfusion, constraints on the extraction-fraction curve. We used (99m)Tc and stannous pyrophosphate for red blood cell imaging to measure intramyocardial blood volume (IMBV) as an alternate measure of microvascular function. RESULTS: The recovery coefficients for myocardial tissue (R(T)) and left ventricular arterial blood (R(A)) were 0.81±0.16 and 1.07±0.12, respectively. The assumption R(T)=1-FBV (Fraction Blood Volume) does not hold in mice. Using a complete mixing matrix to fit a one-compartment model, we measured K1 of 0.57±0.08 min(−1). Constraints on the extraction-fraction curve for (99m)Tc-sestamibi in mice for best-fit Renkin-Crone parameters were α=0.99 and β=0.39. Additionally, we found that wild-type mice increase their IMBV by 22.9±3.3% under hyperemic conditions. CONCLUSIONS: We have developed a framework for measuring K1 and change in IMBV in mice, demonstrating non-invasive μSPECT-based quantitative imaging of mouse microvascular function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink