Enhanced microbiological surveillance reveals that temporal case clusters contribute to the high rates of campylobacteriosis in a model agroecosystem
| Autor: | Vivien V. Suttorp, Andrew L. Webb, G. Douglas Inglis, Eduardo N. Taboada, Valerie F. Boras, Pamela Hodgkinson |
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| Rok vydání: | 2018 |
| Předmět: |
Microbiology (medical)
Agroecosystem Adult Diarrhea Male medicine.medical_specialty Veterinary medicine Adolescent Lari Campylobacteriosis Beef cattle Microbiology Alberta Campylobacter jejuni 03 medical and health sciences Feces Young Adult Epidemiology Campylobacter Infections medicine Prevalence Animals Humans Child 030304 developmental biology Aged Aged 80 and over 0303 health sciences Bacteriological Techniques Molecular epidemiology biology 030306 microbiology Incidence (epidemiology) Outbreak Infant Campylobacter General Medicine Middle Aged biology.organism_classification medicine.disease Infectious Diseases Child Preschool Epidemiological Monitoring Cattle Female Seasons |
| Zdroj: | International journal of medical microbiology : IJMM. 309(3-4) |
| ISSN: | 1618-0607 |
| Popis: | Infections by pathogenic Campylobacter species were determined in diarrheic (n = 2,217) and non-diarrheic control (n = 104) people in Southwestern Alberta (SWA), Canada over a 1-year period using specialized and conventional isolation, and direct PCR. Overall, 9.9% of diarrheic individuals were positive for C. jejuni (9.1%), C. upsaliensis (0.6%), and C. coli (0.5%). No C. lari was detected. Four diarrheic individuals were co-infected with C. jejuni and C. coli, and four different individuals were co-infected with C. jejuni and C. upsaliensis. Two control individuals were positive for C. jejuni. Approximately 50% of stools containing C. jejuni and/or C. coli were deemed negative by conventional isolation. Direct PCR for C. jejuni was less effective than culture-based detection. Most C. jejuni infections occurred in people living in the urban centers, but the prevalence of the bacterium was lower in females than males living in urban locations, and both males and females living in rural locations. Although C. jejuni was detected throughout the year, a trend for higher infection rates was observed in the late spring to early fall with a peak in August. Forty-six C. jejuni subtype clusters were identified, including 44 temporal case clusters attributed to 28 subtype groupings. The majority of infections (70.3%) were linked to subtypes associated with beef cattle. We conclude that many occurrences of pathogenic Campylobacter species were not detected by the conventional laboratory methodology, and temporal case clusters of C. jejuni subtypes associated with cattle contribute to the high rates of campylobacteriosis in SWA. |
| Databáze: | OpenAIRE |
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