RhoA/rock signaling mediates peroxynitrite-induced functional impairment of Rat coronary vessels

Autor: Weiping Li, Zhijun Sun, Hui Peng, Hongwei Li, Xuhua Shen, Xing Wu, L u Ma, Huirong Liu
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
RHOA
Vasodilation
Coronary Artery Disease
030204 cardiovascular system & hematology
Peroxynitrite
Rats
Sprague-Dawley
chemistry.chemical_compound
0302 clinical medicine
ROCK1
Phosphorylation
Cells
Cultured
rho-Associated Kinases
biology
Coronary Vessels
Immunohistochemistry
Cardiology
medicine.symptom
Cardiology and Cardiovascular Medicine
Research Article
Signal Transduction
medicine.medical_specialty
Blotting
Western
Coronary artery
Real-Time Polymerase Chain Reaction
Nitric oxide
Diabetes Mellitus
Experimental
03 medical and health sciences
Internal medicine
Peroxynitrous Acid
medicine
Animals
Kinase activity
business.industry
Rats
Peroxynitrous acid
Disease Models
Animal
030104 developmental biology
Endocrinology
chemistry
Gene Expression Regulation
Vasoconstriction
Hyperglycemia
biology.protein
RhoA/ROCK
RNA
business
rhoA GTP-Binding Protein
Zdroj: BMC Cardiovascular Disorders
ISSN: 1471-2261
Popis: Background Diabetes-induced vascular dysfunction may arise from reduced nitric oxide (NO) availability, following interaction with superoxide to form peroxynitrite. Peroxynitrite can induce formation of 3-nitrotyrosine-modified proteins. RhoA/ROCK signaling is also involved in diabetes-induced vascular dysfunction. The study aimed to investigate possible links between Rho/ROCK signaling, hyperglycemia, and peroxynitrite in small coronary arteries. Methods Rat small coronary arteries were exposed to normal (NG; 5.5 mM) or high (HG; 23 mM) D-glucose. Vascular ring constriction to 3 mM 4-aminopyridine and dilation to 1 μM forskolin were measured. Protein expression (immunohistochemistry and western blot), mRNA expression (real-time PCR), and protein activity (luminescence-based G-LISA and kinase activity spectroscopy assays) of RhoA, ROCK1, and ROCK2 were determined. Results Vascular ring constriction and dilation were smaller in the HG group than in the NG group (P
Databáze: OpenAIRE
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