Reduction of hexavalent chromium by Bacillus spp. isolated from heavy metals-polluted soil
| Autor: | Dora S. Ilić, Hadi Waisi, Ivica Dimkić, Blažo Lalević, Vera Raičević, Saud Hamidović, Panagiotis Gkorezis |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Bacillus safensis
General Chemical Engineering chemistry.chemical_element Bacillus subtilis Bacillus spp bioremediation heavy metal pollution hexavalent chromium reduction indigenous bacteria Bacterial growth chemistry.chemical_compound Chromium Bioremediation Bacillus thuringiensis hexa-valent chromium reduction Food science Hexavalent chromium lcsh:Chemical engineering lcsh:HD9650-9663 biology lcsh:TP155-156 biology.organism_classification chemistry 13. Climate action Bacteria lcsh:Chemical industries |
| Zdroj: | Chemical Industry and Chemical Engineering Quarterly, Vol 25, Iss 3, Pp 247-258 (2019) Chemical Industry and Chemical Engineering Quarterly / CICEQ |
| ISSN: | 2217-7434 1451-9372 |
| Popis: | Hexavalent chromium, Cr(VI), one of the major pollutants from industrial facilities, is very toxic and harmful for human health and environmental quaky. Due to the lack of conventional methods, bioremediation was recommended as an environmentally friendly and effective technique. The aim of this paper was the isolation, identification and selection of the microorganisms which are capable of Cr(VI) reduction in vitro. Heavy metal concentration, detected in four soil samples, within and around the former bicycle factory Wog" (Republic . of Slovenia), was measured using the ICP-OES method. Bacteria were isolated and tested for chromium tolerance using LB agar supplemented with various Cr(VI) concentrations, whilst Cr(VI) reduction and bacterial growth was determined using the LB liquid medium. From 53 bacterial isolates, five of them showed a tolerance of 1000 mg/L of Cr(VI). Those five isolates showed the capability of growth under various Cr(VI) concentrations (50-1000 mg/L). Initial Cr(VI) concentrations ranging from 50 to 100 mg/L were completely reduced by four bacterial isolates, whilst 500 to 1000 mg/L by Bacillus safensis 342-9. Using 16S rDNA and tuf gene sequence analyses, isolates 270-9R and 342-9 were identified as Bacillus safensis, isolates 351-9 and 270-9C as Bacillus subtilis subsp. subtilis, and 212-9 as Bacillus thuringiensis. These results indicated that these bacteria may be promising tools for remediation of metal-polluted sites. The authors acknowledge the partial support of Ministry of Education, Science and Technological Development of Republic of Serbia (Grant Nos. TR31080 and 173026). |
| Databáze: | OpenAIRE |
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