Reduced protein expression of the Na+/Ca2++K+-Exchanger (SLC24A4) in apical plasma membranes of maturation ameloblasts of fluorotic mice
| Autor: | Rozita Jalali, Antonius L.J.J. Bronckers, Jonathan Lytton |
|---|---|
| Přispěvatelé: | ACTA, Orale Celbiologie (ORM, ACTA), Academic Centre for Dentistry Amsterdam, Oral Cell Biology |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Endocrinology Diabetes and Metabolism Enamel fluorosis Transport Antiporters Fluorides 03 medical and health sciences 0302 clinical medicine Endocrinology stomatognathic system Amelogenesis Ameloblasts Animals Orthopedics and Sports Medicine Dental Enamel Original Research Null mutation Enamel paint Chemistry Cell Membrane Sodium Dietary 030206 dentistry Anatomy Apical membrane Cell biology Staining Mice Inbred C57BL Blot Enamel mineralization stomatognathic diseases 030104 developmental biology Membrane visual_art visual_art.visual_art_medium Calcium Mechanism Ameloblast Tooth Calcification Immunostaining |
| Zdroj: | Calcified Tissue International, 100(1), 80-86. Springer New York Calcified Tissue International Bronckers, A L J J, Jalali, R & Lytton, J 2017, ' Reduced protein expression of the Na + /Ca 2+ +K +-Exchanger (SLC24A4) in apical plasma membranes of maturation ameloblasts of fluorotic mice ', Calcified Tissue International, vol. 100, no. 1, pp. 80-86 . https://doi.org/10.1007/s00223-016-0197-4 |
| ISSN: | 0171-967X |
| Popis: | Exposure of forming enamel to fluoride results into formation of hypomineralized enamel. We tested whether enamel hypomineralization was caused by lower expression of the NCKX4/SLC24A4 Ca2+-transporter by ameloblasts. Three commercial antibodies against NCKX4 were tested on enamel organs of wild-type and Nckx4-null mice, one of which (a mouse monoclonal) was specific. This antibody gave a prominent staining of the apical plasma membranes of maturation ameloblasts, starting at early maturation. The layer of immuno-positive ameloblasts contained narrow gaps without immunostaining or with reduced staining. In fluorotic mouse incisors, the quantity of NCKX4 protein in ameloblasts as assessed by western blotting was not different from that in non-fluorotic ameloblasts. However, immunostaining of the apical plasma membranes of fluorotic ameloblasts was strongly reduced or absent suggesting that trafficking of NCKX4 to the apical membrane was strongly reduced. Exposure to fluoride may reduce NCKX4-mediated transport of Ca2+ by maturation stage ameloblasts which delays ameloblast modulation and reduces enamel mineralization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink