cAMP- but not Ca(2+)-regulated Cl- conductance is lacking in cystic fibrosis mice epididymides and seminal vesicles
| Autor: | J. R. Yankaskas, Patrick Y.D. Wong, Anskar Y.H. Leung, R. C. Boucher |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
medicine.medical_specialty Cystic Fibrosis Physiology Cystic fibrosis Mice chemistry.chemical_compound Adenosine Triphosphate Chlorides Reference Values Internal medicine Cyclic AMP medicine Animals Secretion Epididymis Forskolin biology Ionomycin Vesicle Colforsin Electric Conductivity Seminal Vesicles Cell Biology medicine.disease Adenosine Cystic fibrosis transmembrane conductance regulator Mice Inbred C57BL Endocrinology medicine.anatomical_structure chemistry biology.protein Calcium medicine.drug |
| Zdroj: | American Journal of Physiology-Cell Physiology. 271:C188-C193 |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.1996.271.1.c188 |
| Popis: | Cystic fibrosis (CF) reflects the loss of adenosine 3',5'-cyclic monophosphate (cAMP)-regulated Cl- secretion consequent to mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. In humans, but not mice, with CF, the disease is associated with male infertility. The present study investigated the relative magnitudes of the cAMP pathways and an alternative Ca(2+)-regulated Cl- secretory pathway in primary cultures of the epididymides and the seminal vesicles of normal and CF mice. The basal equivalent short-circuit currents (Ieq) of cultures derived from the epididymides and the seminal vesicles from the CF mice were lower (6.0 +/- 0.6 and 4.0 +/- 1.0 muA/cm2, respectively) than those from normal mice (11.1 +/- 1.0 and 6.6 +/- 0.6 muA/cm2, respectively). Forskolin induced significant Ieq responses in both the epididymis (8.0 +/- 0.7 muA/cm2) and seminal vesicles (4.0 +/- 0.5 muA/cm2) from normal mice, whereas forskolin-induced changes in Ieq in CF mouse epididymis and seminal vesicles were absent, consistent with defective cAMP-CFTR-mediated Cl- secretion in CF mice. Ieq responses to agonists (ionomycin, ATP) that raise intracellular Ca2+ (Ca2+i) were larger than forskolin responses in normal animals (6.6 +/- 0.9 and 13.4 +/- 1.8 muA/cm2, respectively) and were preserved in CF (6.5 +/- 0.9 and 17.1 +/- 1.0 muA/cm2, respectively). We speculate that the fertility of male CF mice is maintained by persistent expression of the predominant alternative Ca(2+)-mediated Cl- transport system in the epididymides and seminal vesicles. |
| Databáze: | OpenAIRE |
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