High-Amylose Maize, Potato, and Butyrylated Starch Modulate Large Intestinal Fermentation, Microbial Composition, and Oncogenic miRNA Expression in Rats Fed A High-Protein Meat Diet
| Autor: | Maria L. Marco, Zach Bendiks, Benjamin L. Scherer, Peter Kappel Theil, Tina Skau Nielsen, Matthew E. Wright, Bo Thomsen |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male resistant starch Starch microbiome Maize starch lcsh:Chemistry Rats Sprague-Dawley chemistry.chemical_compound 0302 clinical medicine Food science Resistant starch lcsh:QH301-705.5 Potato starch Spectroscopy Cancer Tumor biology food and beverages General Medicine Computer Science Applications Intestine Colo-Rectal Cancer Butyrates High-Protein 030220 oncology & carcinogenesis Diet High-Protein Colorectal Neoplasms food.ingredient short-chain fatty acids Butyrate Micro-RNA expression Carbohydrate metabolism Zea mays Catalysis Article Inorganic Chemistry Caecum 03 medical and health sciences Short-chain fatty acids food Genetics Biomarkers Tumor Dietary Carbohydrates Animals Intestine Large Physical and Theoretical Chemistry Molecular Biology Nutrition Solanum tuberosum Chemical Physics colon Prevention Organic Chemistry biology.organism_classification butyrate Diet micro-RNA expression Gastrointestinal Microbiome Rats MicroRNAs 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 chemistry Fermentation DNA-adduct Large Sprague-Dawley Amylose Other Biological Sciences Digestive Diseases Other Chemical Sciences Biomarkers |
| Zdroj: | Nielsen, T S, Bendiks, Z, Thomsen, B, Wright, M E, Theil, P K, Scherer, B L & Marco, M L 2019, ' High-Amylose Maize, Potato, and Butyrylated Starch Modulate Large Intestinal Fermentation, Microbial Composition, and Oncogenic miRNA Expression in Rats Fed A High-Protein Meat Diet ', International Journal of Molecular Sciences (Online), vol. 20, no. 9, 2137 . https://doi.org/10.3390/ijms20092137 International Journal of Molecular Sciences International journal of molecular sciences, vol 20, iss 9 International Journal of Molecular Sciences, Vol 20, Iss 9, p 2137 (2019) Volume 20 Issue 9 |
| Popis: | High red meat intake is associated with the risk of colorectal cancer (CRC), whereas dietary fibers, such as resistant starch (RS) seemed to protect against CRC. The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)&mdash produced by an organocatalytic route&mdash could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC. Rats were fed a HPM diet or an HPM diet where 10% of the maize starch was substituted with either HAPS, HAMS, or HAMSB, for 4 weeks. Feces, cecum digesta, and colonic tissue were obtained for biochemical, microbial, gene expression (oncogenic microRNA), and immuno-histochemical (O6-methyl-2-deoxyguanosine (O6MeG) adduct) analysis. The HAMS and HAMSB diets shifted the fecal fermentation pattern from protein towards carbohydrate metabolism. The HAMSB diet also substantially increased fecal butyrate concentration and the pool, compared with the other diets. All three RS treatments altered the cecal microbial composition in a diet specific manner. HAPS and HAMSB showed CRC preventive effects, based on the reduced colonic oncogenic miR17-92 cluster miRNA expression, but there was no significant diet-induced differences in the colonic O6MeG adduct levels. Overall, HAMSB consumption showed the most potential for limiting the negative effects of a high-meat diet. |
| Databáze: | OpenAIRE |
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