A novel MDMX transcript expressed in a variety of transformed cell lines encodes a truncated protein with potent p53 repressive activity
| Autor: | B Cho, W E Mercer, Gordon D. Strachan, David J. Hall, Ravikumar Rallapalli |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
MDMX
Transcription Genetic Molecular Sequence Data Biology Transfection Biochemistry Cell Line Gene product Mice Open Reading Frames Transcription (biology) Proto-Oncogene Proteins Animals Amino Acid Sequence Nuclear protein Fluorescent Antibody Technique Indirect Molecular Biology Gene Peptide sequence Base Sequence Nuclear Proteins Proto-Oncogene Proteins c-mdm2 Cell Biology 3T3 Cells Molecular biology Stop codon Peptide Fragments Cell biology Repressor Proteins Open reading frame Tumor Suppressor Protein p53 |
| Zdroj: | The Journal of biological chemistry. 274(12) |
| ISSN: | 0021-9258 |
| Popis: | The MDMX gene product is related to the MDM2 oncoprotein, both of which interact with the p53 tumor suppressor. We have identified a novel transcript of the MDMX gene that is expressed in a variety of cell lines, and in particular, in growing and transformed cells. This transcript is identical to the published sequence yet it has a short internal deletion of 68 base pairs. This deletion produces a shift in the reading frame after codon 114, resulting in the inclusion of a stop codon at amino acid residue 127 (full-length MDMX is 489 residues). This truncated MDMX protein is termed MDMX-S ("short form"), represents only the p53-binding domain, and appears to bind p53 better than full-length MDMX. The MDMX-S protein can be detected in cell extracts and when overexpressed is much more effective than MDMX at inhibiting p53-mediated transcriptional activation and induction of apoptosis. Since MDMX-S lacks the central and carboxyl-terminal regions contained within full-length MDMX, it is likely to play a key role in the regulation of cell proliferation and apoptosis in a way distinct from MDMX. |
| Databáze: | OpenAIRE |
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