Influence of poly A-poly U on early events in the immune response in vitro
| Autor: | Librado Ortiz-Ortiz, Bernard N. Jaroslow |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Erythrocytes
Uracil Nucleotides medicine.medical_treatment Polynucleotides Immunology Bone Marrow Cells Mice Inbred Strains Thymus Gland In Vitro Techniques Biology Mice Tissue culture Ribonucleases Immune system Bone Marrow In vivo medicine Animals Antibody-Producing Cells Cells Cultured Deoxyribonucleases Sheep Adenine Nucleotides RNA DNA Molecular biology Stimulation Chemical In vitro Kinetics Biochemistry Polynucleotide Antibody Formation Nucleic acid Female Adjuvant Spleen |
| Zdroj: | Cellular Immunology. 3:123-132 |
| ISSN: | 0008-8749 |
| DOI: | 10.1016/0008-8749(72)90232-8 |
| Popis: | Adjuvant action of artificial homopolyribonucleotides and nucleic acid digests was studied in mouse spleen-cell cultures, stimulated with sheep red cells by the technique of Mishell and Dutton. Assays of antibody-forming cells were performed according to the Jerne and Nordin technique. Doublestranded poly A-poly U, and RNA digests had adjuvant activity over a wide dose range (0.01–100 μg/ml). DNA digests, single stranded polynucleotides (poly A, poly U, poly C) and mixtures of ribonucleosides and ribonucleotides had little or no enhancing effect at comparable doses. From our study of the kinetics of increase of plaque-forming cells in cultures treated with poly A-poly U, in conjunction with in vivo studies of others, we concluded that adjuvant action, during induction, indirectly increased the number of bone marrow-derived cells that responded to antigenic stimulation by increasing the effectiveness of the thymus-derived cells in the culture. |
| Databáze: | OpenAIRE |
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