Effect of Hepatic Stimulator Substance (HSS) on Cadmium-Induced Acute Hepatotoxicity in the Rat Liver
| Autor: | Georgios I Panoutsopoulos, Katerina N. Alexandropoulou, Rosa I. Hereti, Konstantinos N. Tzirogiannis, Michael G. Mykoniatis, Maria D. Demonakou |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Thymidine kinase activity Programmed cell death Pathology Necrosis Physiology Apoptosis Biology Internal medicine medicine Animals Peliosis Hepatis Rats Wistar Growth Substances Liver injury integumentary system Gastroenterology medicine.disease Liver regeneration Liver Regeneration Rats Endocrinology medicine.anatomical_structure Liver Hepatocyte Acute Disease Toxicity Hepatocytes Intercellular Signaling Peptides and Proteins Mitogens medicine.symptom Peptides |
| Zdroj: | Digestive Diseases and Sciences. 49:1019-1028 |
| ISSN: | 0163-2116 |
| DOI: | 10.1023/b:ddas.0000034566.43582.53 |
| Popis: | The hepatoprotective effect of HSS against cadmium-induced liver injury was investigated. Rats were intoxicated with a dose of cadmium (3.5 mg/kg b.w.). The rats were treated with normal saline (group I) or HSS (100 mg protein/kg b.w.; group II) 2 hr later and killed at different time points. Hematoxylin-eosin (HE) sections were assessed for necrosis, apoptosis, peliosis, mitoses, and inflammatory infiltration. Serum enzyme activities were assayed. Apoptosis was quantified by the Tunel technique. Thymidine kinase activity and the rate of [3H]thymidine incorporation into DNA were also assayed. Necrosis, hepatocyte apoptosis, and peliosis were minimized in HSS-treated rats (group II). Nonparenchymal cell apoptosis and liver regeneration were not quantitively altered in the HSS-treated group, though the time profile was different. HSS protects hepatocytes against cadmium-induced necrosis, apoptosis, and peliosis. Apoptosis was the major type of cell death for nonparenchymal liver cells and strongly correlated with the extent of peliosis. Interactions between hepatocytes and nonparenchymal liver cells seem to be important for the genesis of hepatic trauma in acute cadmium hepatotoxicity. |
| Databáze: | OpenAIRE |
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