Cross-linked enzyme aggregates of alginate lyase: A systematic engineered approach to controlled degradation of alginate hydrogel
| Autor: | Abhijit Roy, Sudhanshu Shekhar, Sangeetha Kunjukunju, Prashant N. Kumta |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Ammonium sulfate
Immobilized enzyme Cross-linked enzyme aggregate Alginates 02 engineering and technology 01 natural sciences Biochemistry Flavobacterium Catalysis chemistry.chemical_compound Engineering Glucuronic Acid Structural Biology Enzyme Stability Animals Molecular Biology Polysaccharide-Lyases chemistry.chemical_classification biology 010405 organic chemistry Hexuronic Acids General Medicine 021001 nanoscience & nanotechnology Enzymes Immobilized Enzyme assay 0104 chemical sciences Enzyme chemistry Chemical engineering Glutaral biology.protein Solvents Degradation (geology) Glutaraldehyde 0210 nano-technology |
| Zdroj: | International journal of biological macromolecules. 115 |
| ISSN: | 1879-0003 |
| Popis: | An enzyme aggregate of alginate lyase (EC 4.2.2.3) from flavobactierium was prepared using ammonium sulfate. The resultant aggregates upon cross-linking with glutaraldehyde produced insoluble and catalytically active cross-linked enzyme aggregate (CLEA) enzyme. The catalytic activity and stability of the cross-linked enzyme aggregate of alginate lyase (CLEA-AL) was studied in the presence of various pH, temperatures and organic solvents. Reusability, storage stability and surface morphology of the CLEA-AL were also studied. The native enzyme and CLEA-AL exhibited maximum enzyme activity at pH of 6.3 and at a temperature of 40°C. The CLEA-AL has good stability in nonpolar organic solvents and is thermally stable up to 50°C over a period of 8h. By encapsulating CLEA-AL into alginate hydrogel, we demonstrate that alginate hydrogels can be enzymatically degraded in a controlled fashion. The results also showed that degradation of alginate hydrogel with CLEA-AL incorporated beads is slower than native enzyme and therefore, CLEA-AL can be used for controlled degradation and release of various biologics from the degrading gel. |
| Databáze: | OpenAIRE |
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