Oral administration activity determination of recombinant osteoprotegerin from silkworm larvae
| Autor: | Hai-Long Xiao, Xiang-Fu Wu, Yao-Zhou Zhang |
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| Rok vydání: | 2007 |
| Předmět: |
Male
musculoskeletal diseases medicine.medical_specialty medicine.drug_class Ovariectomy Administration Oral Bioengineering Biology Applied Microbiology and Biotechnology Biochemistry Bone resorption law.invention Mice Osteoprotegerin Oral administration Bombyx mori law Internal medicine medicine Animals Humans Molecular Biology Mice Inbred ICR fungi Biological activity Bombyx biology.organism_classification Recombinant Proteins Endocrinology Estrogen Larva Ovariectomized rat Recombinant DNA Calcium Female Bone Remodeling Biotechnology |
| Zdroj: | Molecular Biotechnology. 35:179-184 |
| ISSN: | 1559-0305 1073-6085 |
| DOI: | 10.1007/bf02686103 |
| Popis: | Osteoprotegerin (OPG) regulates the formation of osteoclasts and is involved in the regulation of bone resorption and remodeling. To investigate the feasibility of using silkworm (Bombyx mori) larvae to produce recombinant osteoprotegerin as a oral administration drug, the rh-OPG was expressed in the larvae of silkworm through the silkworm baculovirus expression system, and was orally administered to mice. Compared with the control, oral administration of rh-OPG was effective to decrease serum calcium concentration in normal mice, and block the bone loss induced by the loss of estrogen in ovariectomized mice. These results indicated that oral administration of rh-OPG expressed in silkworm larvae had the proper bioactivity. |
| Databáze: | OpenAIRE |
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