Hepatoprotective effect of Omega-3 PUFAs against acute paracetamol-induced hepatic injury confirmed by FTIR

Autor: Sah Youssef, Seham A. El-Batran, Zeinab A. El-Gendy, Rofanda M. Bakeer, Rania F. Ahmed, Walid El Hotaby, A. Ramadan
Rok vydání: 2020
Předmět:
0301 basic medicine
Antioxidant
Health
Toxicology and Mutagenesis
medicine.medical_treatment
Pharmacology
Toxicology
Nitric Oxide
Protective Agents
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Malondialdehyde
Fatty Acids
Omega-3
Spectroscopy
Fourier Transform Infrared
medicine
Animals
Aspartate Aminotransferases
Hepatoprotective Agent
Rats
Wistar
Acetaminophen
Liver injury
chemistry.chemical_classification
Cholesterol
business.industry
Interleukin-6
Tumor Necrosis Factor-alpha
Low dose
Alanine Transaminase
General Medicine
Glutathione
Analgesics
Non-Narcotic
medicine.disease
030104 developmental biology
Oral ingestion
chemistry
Liver
030220 oncology & carcinogenesis
Hepatocytes
lipids (amino acids
peptides
and proteins)
Female
Chemical and Drug Induced Liver Injury
business
Polyunsaturated fatty acid
Zdroj: Humanexperimental toxicology. 40(3)
ISSN: 1477-0903
Popis: Acute paracetamol over dose-induced hepatotoxicity is considered an important medical hazard especially among women. Omega-3 long-chain polyunsaturated fatty acids (Omega-3 PUFAs) daily doses are nowadays recommended for their antioxidant and anti-inflammatory potentials. Fourier transform infrared (FTIR) spectroscopy is considered a reliable method in analyzing cellular alterations and is now efficiently used to diagnose several diseases and the efficacy of drugs even in the early stages. The aim of our study was to evaluate the hepatoprotective effect of Omega-3 PUFAs against paracetamol-induced hepatotoxicity in rats confirmed through measuring protein alterations in hepatocytes by FTIR. Rats were pretreated with Omega-3 PUFAs (50 and 100 mg/kg) for 21 days prior to oral ingestion of paracetamol. FTIR results revealed that Omega-3 PUFAs (50 mg/kg) limited the toxic effects of paracetamol by restoring the hepatic amide I to amide II ratio. In addition; biochemical analyses demonstrated that serum ALT, AST, Cholesterol, LDL-cholesterol and Il-6 levels as well as hepatic TNF-α, MDA, NOx levels were decreased. Besides; serum HDL-cholesterol level and hepatic GSH level were increased. Histopathological examinations of hepatic sections validated the hepatoprotective potential. The overall effect of this dose was comparable to those of the usual recommended hepatoprotective supplement; silymarin. In conclusion; it would be recommended to use Omega-3 PUFAs in low doses on daily bases as a hepatoprotective agent.
Databáze: OpenAIRE
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