Bioremediation of hydrocarbon degradation in a petroleum-contaminated soil and microbial population and activity determination
| Autor: | Kaili Chen, David Kost, Xi-Qiong Ye, Liming Chen, Warren A. Dick, Manli Wu, Wei Li |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Environmental Engineering Health Toxicology and Mutagenesis Microorganism Population 010501 environmental sciences complex mixtures 01 natural sciences 03 medical and health sciences Soil Bioremediation Most probable number Alkanes Environmental Chemistry Soil Pollutants Petroleum Pollution Polycyclic Aromatic Hydrocarbons education Soil Microbiology 0105 earth and related environmental sciences Alkane chemistry.chemical_classification education.field_of_study Public Health Environmental and Occupational Health General Medicine General Chemistry Pollution Soil contamination Hydrocarbons 030104 developmental biology Hydrocarbon Biodegradation Environmental Petroleum chemistry Environmental chemistry Microcosm |
| Zdroj: | Chemosphere. 169 |
| ISSN: | 1879-1298 |
| Popis: | Bioremediation of hydrocarbon degradation in petroleum-polluted soil is carried out by various microorganisms. However, little information is available for the relationships between hydrocarbon degradation rates in petroleum-contaminated soil and microbial population and activity in laboratory assay. In a microcosm study, degradation rate and efficiency of total petroleum hydrocarbons (TPH), alkanes, and polycyclic aromatic hydrocarbons (PAH) in a petroleum-contaminated soil were determined using an infrared photometer oil content analyzer and a gas chromatography mass spectrometry (GC-MS). Also, the populations of TPH, alkane, and PAH degraders were enumerated by a modified most probable number (MPN) procedure, and the hydrocarbon degrading activities of these degraders were determined by the Biolog (MT2) MicroPlates assay. Results showed linear correlations between the TPH and alkane degradation rates and the population and activity increases of TPH and alkane degraders, but no correlation was observed between the PAH degradation rates and the PAH population and activity increases. Petroleum hydrocarbon degrading microbial population measured by MPN was significantly correlated with metabolic activity in the Biolog assay. The results suggest that the MPN procedure and the Biolog assay are efficient methods for assessing the rates of TPH and alkane, but not PAH, bioremediation in oil-contaminated soil in laboratory. |
| Databáze: | OpenAIRE |
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