Amperometric biosensor based on Laccase immobilized onto a screen-printed electrode by Matrix Assisted Pulsed Laser Evaporation
| Autor: | Maria Dinescu, Marius Dumitru, Antonio Morone, Nunzia Cicco, Fabio Favati, Diego Centonze, Fabiana Crispo, Maria Verrastro |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Immobilized enzyme
Biosensing Techniques 02 engineering and technology Biosensor Matrix AssistedPulsedLaserEvaporation Screen PrintedElectrode Laccase Polyphenols assessment 01 natural sciences Analytical Chemistry Matrix Assisted Pulsed Laser Evaporation Matrix (chemical analysis) Polyphenols assessment Electrodes Detection limit Laccase Chromatography Aqueous solution Chemistry 010401 analytical chemistry Reproducibility of Results Screen Printed Electrode Enzymes Immobilized 021001 nanoscience & nanotechnology Matrix AssistedPulsedLaserEvaporation 0104 chemical sciences Linear range Electrode Volatilization 0210 nano-technology Biosensor Screen PrintedElectrode |
| Zdroj: | Talanta (Oxf.) 154 (2016): 438–445. doi:10.1016/j.talanta.2016.03.072 info:cnr-pdr/source/autori:Verrastro M.; Cicco N.; Crispo F.; Morone A.; Dinescu M.; Dumitru M.; Favati F.; Centonze D./titolo:Amperometric biosensor based on Laccase immobilized onto a screen-printed electrode by Matrix Assisted Pulsed Laser Evaporation/doi:10.1016%2Fj.talanta.2016.03.072/rivista:Talanta (Oxf.)/anno:2016/pagina_da:438/pagina_a:445/intervallo_pagine:438–445/volume:154 |
| DOI: | 10.1016/j.talanta.2016.03.072 |
| Popis: | A Laccase-based biosensor for the determination of phenolic compounds was developed by using Matrix Assisted Pulsed Laser Evaporation as an innovative enzyme immobilization technique. and the deriving biosensor was characterized and applied for the first time. Laccase was immobilized onto different substrates including screen printed carbon electrodes and spectroscopic, morphologic and electrochemical characterizations were carried out. A linear range from 1 to 60 μM was achieved working at 5.5 pH and −0.2 V detection potential vs Ag pseudoreference. The limits of detection and quantification were found to be 1 and 5 μM, respectively. A good fabrication reproducibility, stability of response and selectivity toward interferents were also found The potential of the developed biosensor was tested in the determination of total polyphenol content in real matrices (tea infusion, ethanolic extract from Muscari comosum bulbs and aqueous solution of a food supplement from black radish root and artichoke leaves) and the results were compared with those obtained by using the Folin–Ciocalteu method. |
| Databáze: | OpenAIRE |
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