Activity of taraxasteryl acetate on inflammation and heat shock protein synthesis

Autor: Teodor Parella, Salvador Cañigueral, Esther Marín, Tomás Adzet, Francisco Pérez-García
Rok vydání: 2005
Předmět:
Male
Croton Oil
Neutrophils
Pharmaceutical Science
Inflammation
HSP72 Heat-Shock Proteins
Mice
Inbred Strains
Pharmacology
Asteraceae
medicine.disease_cause
Rats
Sprague-Dawley
chemistry.chemical_compound
Mice
Heat shock protein
Drug Discovery
medicine
Bioassay
Animals
Edema
Humans
Croton oil
Rats
Wistar
Reactive nitrogen species
Heat-Shock Proteins
chemistry.chemical_classification
Reactive oxygen species
Arachidonic Acid
Dose-Response Relationship
Drug
Plant Extracts
Granuloma
Foreign-Body
Anti-Inflammatory Agents
Non-Steroidal
Flow Cytometry
Reactive Nitrogen Species
Triterpenes
Rats
Sterols
Complementary and alternative medicine
chemistry
Biochemistry
Molecular Medicine
Tetradecanoylphorbol Acetate
Arachidonic acid
Female
medicine.symptom
Plant Structures
Reactive Oxygen Species
Oxidative stress
Phytotherapy
Zdroj: Phytomedicine : international journal of phytotherapy and phytopharmacology. 12(4)
ISSN: 0944-7113
Popis: Pluchea sagittalis whole plant dichloromethane extract showed inhibitory activity in several inflammatory models: rat hind paw-edema, mice ear edema, and air-pouch rat granuloma. The extract inhibited the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in stimulated human neutrophils. It also showed inhibitory effect on heat shock protein 72 (hsp72) synthesis in stimulated neutrophils, while it had opposite effects on unstimulated cells. The triterpene taraxasteryl acetate was obtained from the dichloromethane extract by bioassay directed isolation, being active against induced ROS and RNS production in human neutrophils. In mice ear edema (induced by phorbol-12-mirystate-13-acetate, croton oil and arachidonic acid), taraxasteryl acetate showed a topical anti-inflammatory activity similar to the extract, but at 1/20 of the dose. The same ratio was observed for the inhibition of hsp72 production in stimulated human neutrophils. In unstimulated monocytes and neutrophils, taraxasteryl acetate showed a higher stimulating activity of hsp72 production than the extract, involving different mechanisms in each cell type. To our knowledge, taraxasteryl acetate is the first natural product for which a dual effect on the hsp response is reported.
Databáze: OpenAIRE
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