Mutual promotions between periodontitis and vascular calcification by rat animal model
| Autor: | Pengmei Zhang, Ke-qing Pan, Shu Li, Yun Meng, Wenbin Song, Jing Deng, Huixu Li |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Alveolar Bone Loss H&E stain 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Animals Osteopontin Rats Wistar Vascular Calcification Vascular tissue Inflammation Periodontitis biology business.industry 030206 dentistry medicine.disease Chronic periodontitis Rats Resorption Disease Models Animal 030104 developmental biology Endocrinology biology.protein Periodontics Alkaline phosphatase business Calcification |
| Zdroj: | Journal of Periodontal Research. 55:810-820 |
| ISSN: | 1600-0765 0022-3484 |
| DOI: | 10.1111/jre.12757 |
| Popis: | OBJECTIVE AND BACKGROUND To study the relationship between periodontitis and vascular calcification by establishing rat model of chronic periodontitis and vascular calcification. METHODS Forty male Wistar rats were divided into four groups randomly: control group, periodontitis group, vascular calcification group, and compound periodontitis and calcification group. Each group rats accepted the corresponding manages to establish the animal model. Clinical examinations and hematoxylin and eosin staining of periodontal tissue were taken to test the periodontal model; calcium assay, alkaline phosphatase activity, expression of mineral-related factors including osteopontin, alkaline phosphatase, core-binding factor-α1 and bone sialoprotein, hematoxylin and eosin staining and von Kossa staining of vascular tissue were taken to test the vascular calcification model; inflammatory factors including C-reactive protein, interleukin-1β, tumor necrosis factor-α, interleukin-6, prostaglandin E2, and serum lipid in serum were also detected at the same time. RESULTS The rat model was established. Inflammation of periodontal tissue and alveolar bone resorption in compound group and periodontitis group were more obvious than those in control group and vascular calcification group (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text