Effects of aerobic exercise training on muscle plasticity in a mouse model of cervical spinal cord injury
| Autor: | Alexia Paucard, Therese B. Deramaudt, Stéphane Vinit, Pauline Michel-Flutot, Marcel Bonay, Isley Jesus, Valentin Vanhee |
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| Přispěvatelé: | Handicap neuromusculaire : Physiopathologie, Biothérapie et Pharmacologies appliquées (END-ICAP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale, Inserm, Chancellerie des Universités de Paris, This work was supported by funding from the Chancellerie des Universités de Paris (Legs Poix) (SV, MB), INSERM (MB), Université de Versailles Saint-Quentin-en-Yvelines (MB, SV). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript., Hôpital Ambroise Paré [AP-HP], HAL UVSQ, Équipe |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Cell biology Physiology Science [SDV]Life Sciences [q-bio] Diaphragmatic breathing Diseases Electromyography Article Incremental exercise 03 medical and health sciences 0302 clinical medicine medicine Respiratory muscle Animals Humans Myocyte Aerobic exercise Respiratory system Exercise Spinal Cord Injuries Multidisciplinary medicine.diagnostic_test business.industry Muscles Cervical Cord Recovery of Function 3. Good health Diaphragm (structural system) [SDV] Life Sciences [q-bio] Mice Inbred C57BL Disease Models Animal 030104 developmental biology Anesthesia Medicine Female business 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2021, 11 (1), ⟨10.1038/s41598-020-80478-9⟩ Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-80478-9⟩ |
| Popis: | Cervical spinal cord injury (SCI) results in permanent life-altering motor and respiratory deficits. Other than mechanical ventilation for respiratory insufficiency secondary to cervical SCI, effective treatments are lacking and the development of animal models to explore new therapeutic strategies are needed. The aim of this work was to demonstrate the feasibility of using a mouse model of partial cervical spinal hemisection at the second cervical metameric segment (C2) to investigate the impact of 6 weeks training on forced exercise wheel system on locomotor/respiratory plasticity muscles. To measure run capacity locomotor and respiratory functions, incremental exercise tests and diaphragmatic electromyography were done. In addition, muscle fiber type composition and capillary distribution were assessed at 51 days following chronic C2 injury in diaphragm, extensor digitorum communis (EDC), tibialis anterior (TA) and soleus (SOL) muscles. Six-week exercise training increased the running capacity of trained SCI mice. Fiber type composition in EDC, TA and SOL muscles was not modified by our protocol of exercise. The vascularization was increased in all muscle limbs in SCI trained group. No increase in diaphragmatic electromyography amplitude of the diaphragm muscle on the side of SCI was observed, while the contraction duration was significantly decreased in sedentary group compared to trained group. Cross-sectional area of type IIa myofiber in the contralateral diaphragm side of SCI was smaller in trained group. Fiber type distribution between contralateral and ipsilateral diaphragm in SCI sedentary group was affected, while no difference was observed in trained group. In addition, the vascularization of the diaphragm side contralateral to SCI was increased in trained group. All these results suggest an increase in fatigue resistance and a contribution to the running capacity in SCI trained group. Our exercise protocol could be a promising non-invasive strategy to sustain locomotor and respiratory muscle plasticity following SCI. |
| Databáze: | OpenAIRE |
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