Histological and biochemical evaluation of plasma rich in growth factors treatment for grade II muscle injuries in sheep

Autor:	Daniel Aguilar-García, J. Andrés Fernández-Sarmiento, María del Mar Granados Machuca, Juan Morgaz Rodríguez, Pilar Muñoz Rascón, Rocío Navarrete Calvo, Yolanda Millán Ruiz, José María Carrillo Poveda, Juan Muñoz Castañeda, Ramón Cugat Bertomeu, Juan Manuel Domínguez Pérez
Přispěvatelé:	Producción Científica UCH 2022, UCH. Departamento de Medicina y Cirugía Animal, UCH. Cátedra Fundación García Cugat
Rok vydání:	2022
Předmět:	Muscles - Wounds and injuries - Treatment Growth factors - Therapeutic use Sheep General Veterinary Platelet-Rich Plasma Muscles General Medicine Plasma rich in growth factors Plasma Platelet-rich plasma Euthanasia Animal Muscle repair Animals Intercellular Signaling Peptides and Proteins Crecimiento - Factores - Uso terapéutico Muscle injury Single-Blind Method Plasma sanguíneo - Uso terapéutico Blood plasma - Therapeutic use Músculos - Heridas y lesiones - Tratamiento
Zdroj:	BMC veterinary research. 18(1)
ISSN:	1746-6148
Popis:	Este artículo se encuentra disponible en la siguiente URL: https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-022-03491-2 En este artículo de investigación también participan: Yolanda Millán Ruiz, Juan Muñoz Castañeda, Ramón Cugat Bertomeu y Juan Manuel Domínguez Pérez. The purpose of this study was to perform a histological and biochemical evaluation of the influence of plasma rich in growth factors (PRGF) on muscle regeneration process after a surgically induced grade II muscle laceration. A randomized, single blind, controlled experimental research was conducted including twenty-one adult healthy sheep, randomly divided in three groups (n = 7). A grade II surgical section was performed in the biceps femoris muscle of both hindlimbs. After two days (basal time), intralesional infiltration of autologous PRGF or Saline solution was randomly administered in both hindlimbs. Treatment was repeated once a week. Animal groups were euthanized at 1 (T1), 2 (T2) or 4 (T4) weeks. Histological assessment showed that PRGF intralesional injection induced a significant decrease of inflammatory cells density, significant higher centrally nucleated fibers percentage and significantly smaller fibrotic areas compared to Saline-treated muscles at T1, T2 and T4. Also, lower vascular density, with lower capillaries cross-sectional area, in PRGF group compared to Saline was observed. Biochemical analysis revealed a significant higher expression level of MYOD1, MYF5 and MYOG genes in PRGF groups at T1 compared to Saline treated muscles. At ultrastructural level, PRGF groups presented scarce edema and loss of connective tissue structure, as well as higher mitochondrial density adequately associated to the sarcomere unit in contrast to the Saline group. In conclusion, histological, biochemical, and ultrastructural results showed that PRGF treatment improved muscle regeneration process leading to more mature histological aspect in newly formed muscle tissue after a surgically induced grade II muscle injury.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd0568a33b9dcd366230a4a13b3bdf05 https://pubmed.ncbi.nlm.nih.gov/36371198 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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