Impact of β-hydroxy β-methylbutyrate (HMB) on age-related functional deficits in mice
| Autor: | Tor W. Jensen, Adam Cobert, Ryan Brander, Michael Munroe, Marni D. Boppart, Yair Pincu, Justin S. Rhodes, Jennifer R. Merritt |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Aging medicine.medical_specialty Stromal cell Neurogenesis Metabolite Hippocampal formation Biology Biochemistry Mice 03 medical and health sciences chemistry.chemical_compound Cognition 0302 clinical medicine Endocrinology Internal medicine Gene expression Valerates Genetics medicine Animals Muscle Strength Muscle Skeletal skin and connective tissue diseases Molecular Biology Essential amino acid chemistry.chemical_classification Body Weight Mesenchymal stem cell Mesenchymal Stem Cells Cell Biology Mice Inbred C57BL 030104 developmental biology chemistry Dietary Supplements Female Leucine Transcriptome human activities 030217 neurology & neurosurgery |
| Zdroj: | Experimental Gerontology. 87:57-66 |
| ISSN: | 0531-5565 |
| DOI: | 10.1016/j.exger.2016.11.010 |
| Popis: | β-Hydroxy β-methylbutyrate (HMB) is a metabolite of the essential amino acid leucine. Recent studies demonstrate a decline in plasma HMB concentrations in humans across the lifespan, and HMB supplementation may be able to preserve muscle mass and strength in older adults. However, the impact of HMB supplementation on hippocampal neurogenesis and cognition remains largely unexplored. The purpose of this study was to simultaneously evaluate the impact of HMB on muscle strength, neurogenesis and cognition in young and aged mice. In addition, we evaluated the influence of HMB on muscle-resident mesenchymal stem/stromal cell (Sca-1+CD45-; mMSC) function to address these cells potential to regulate physiological outcomes. Three month-old (n=20) and 24 month-old (n=18) female C57BL/6 mice were provided with either Ca-HMB or Ca-Lactate in a sucrose solution twice per day for 5.5weeks at a dose of 450mg/kg body weight. Significant decreases in relative peak and mean force, balance, and neurogenesis were observed in aged mice compared to young (age main effects, p≤0.05). Short-term HMB supplementation did not alter activity, balance, neurogenesis, or cognitive function in young or aged mice, yet HMB preserved relative peak force in aged mice. mMSC gene expression was significantly reduced with age, but HMB supplementation was able to recover expression of select growth factors known to stimulate muscle repair (HGF, LIF). Overall, our findings demonstrate that while short-term HMB supplementation does not appear to affect neurogenesis or cognitive function in young or aged mice, HMB may maintain muscle strength in aged mice in a manner dependent on mMSC function. |
| Databáze: | OpenAIRE |
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