Feasibility of dried blood spots for HIV viral load monitoring in decentralized area in North Vietnam in a test-and-treat era, the MOVIDA project
| Autor: | Tuan Anh Nguyen, Tram Hong Tran, Binh Thanh Nguyen, Tram Thi Phuong Pham, Nhung Thi Hong Le, Dung Viet Ta, Huong Thi Thu Phan, Long Hoang Nguyen, Mohand Ait-Ahmed, Hien Thi Ho, Fabien Taieb, Yoann Madec, MOVIDA 2 study group |
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| Přispěvatelé: | National Institute of Hygiene and Epidemiology [Hanoi, Vietnam] (NIHE), Réseau International des Instituts Pasteur (RIIP), Hanoi University of Public Health (HUPH), Recherches Translationnelles sur le VIH et les maladies infectieuses (TransVIHMI), Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Universtié Yaoundé 1 [Cameroun]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Epidémiologie des Maladies Emergentes - Emerging Diseases Epidemiology, Pasteur-Cnam risques infectieux et émergents (PACRI), Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris], Ministry of Health [Hanoi], Centre de Recherche Translationnelle - Center for Translational Science (CRT), Institut Pasteur [Paris] (IP), Pasteur-Cnam Risques infectieux et émergents (PACRI), Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Institut Pasteur [Paris] (IP)-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), The study was funded by the Global Fund to figth AIDS malaria and tuberculosis and by the Ministry of Health of Vietnam. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript., Institut Pasteur [Paris], Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut Pasteur [Paris]-Conservatoire National des Arts et Métiers [CNAM] (CNAM) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
RNA viruses
Male 0301 basic medicine Research Facilities Time Factors Physiology Epidemiology HIV Infections Drug resistance Pathology and Laboratory Medicine Geographical Locations Cohort Studies 0302 clinical medicine Immunodeficiency Viruses Interquartile range [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Medicine and Health Sciences Medicine Public and Occupational Health Prospective Studies Treatment Failure 030212 general & internal medicine Dried blood Prospective cohort study Multidisciplinary Viral Load Middle Aged Vaccination and Immunization Clinical Laboratory Sciences Body Fluids 3. Good health Clinical Laboratories Blood Vietnam Anti-Retroviral Agents Medical Microbiology HIV epidemiology Viral Pathogens Viruses Female Pathogens Anatomy Research Laboratories Viral load HIV drug resistance Research Article Cohort study Adult medicine.medical_specialty Asia Science Immunology Antiretroviral Therapy Research and Analysis Methods Microbiology 03 medical and health sciences Antiviral Therapy Diagnostic Medicine Virology Retroviruses Drug Resistance Viral Humans Microbial Pathogens business.industry Lentivirus Organisms Biology and Life Sciences HIV 030112 virology People and Places Emergency medicine HIV-1 Feasibility Studies Observational study [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie Preventive Medicine Dried Blood Spot Testing business Viral Transmission and Infection Government Laboratories |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2020, 15 (4), pp.e0230968. ⟨10.1371/journal.pone.0230968⟩ PLoS ONE, 2020, 15 (4), pp.e0230968. ⟨10.1371/journal.pone.0230968⟩ PLoS ONE, Vol 15, Iss 4, p e0230968 (2020) |
| ISSN: | 1932-6203 |
| Popis: | International audience; Background: Access to HIV viral load is crucial to efficiently monitor patients on antiretroviral treatment (ART) and prevent HIV drug resistance acquisition. However, in some remote settings, access to viral load monitoring is still complex due to logistical and financial constraints. Use of dried blood spots (DBS) for blood collection could overcome these difficulties. This study aims to describe feasibility and operability of DBS use for routine viral load monitoring.Methods: From June 2017 to April 2018, HIV-infected adults who initiated ART were enrolled in a prospective cohort in 43 clinical sites across 6 provinces in North Vietnam. Following national guidelines, the first viral load monitoring was planned 6 months after ART initiation. DBS were collected at the clinical site and sent by post to a central laboratory in Hanoi for viral load measurement.Results: Of the 578 patients enrolled, 537 were still followed 6 months after ART initiation, of which DBS was collected for 397 (73.9%). The median (inter quartile range) delay between DBS collection at site level and reception at the central laboratory was 8 (6–19) days and for 70.0% viral load was measured ≤30 days after blood collection. The proportion of patients with viral load ≥1000 copies/mL at the 6 month evaluation was 15.9% (n = 59). Of these, a DBS was collected again to confirm virological failure in 15 (24.4%) of which virological failure was confirmed in 11 (73.3%).Conclusion: Delay of DBS transfer to the central laboratory was acceptable and most viral loads were measured in ≤30 days, in-line with routine follow-up. However, the level of DBS coverage and the proportion of patients in failure for whom a confirmatory viral load was available were suboptimal, indicating that integration of viral load monitoring in the field requires, among other things, careful training and strong involvement of the local teams. The proportion of patients experiencing virological failure was in line with other reports; interestingly those who reported being non-adherent and those with a low BMI were more at risk of failure |
| Databáze: | OpenAIRE |
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