2- Isopenteniladenina na capacidade de indução da embriogênese somática direta de Coffea arabica L
| Autor: | Valéria Cristina Barbosa Carmazini, Ivanilda dos Santos Alves, Cosme Damião dos Santos, Julieta Andrea Silva de Almeida |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Somatic embryogenesis Palavras chave embriões somáticos 02 engineering and technology Biology 01 natural sciences lcsh:Agriculture 0202 electrical engineering electronic engineering information engineering Cultivar lcsh:Agriculture (General) 2-iP General Veterinary Coffea arabica lcsh:S embryogenic structures somatic embryos estrutura embriogênica lcsh:S1-972 Horticulture Micropropagation 020201 artificial intelligence & image processing Animal Science and Zoology Agronomy and Crop Science 010606 plant biology & botany Explant culture Cultivar Mundo Novo |
| Zdroj: | Ciência Rural, Vol 48, Iss 11 (2018) Ciência Rural v.48 n.11 2018 Ciência Rural Universidade Federal de Santa Maria (UFSM) instacron:UFSM Ciência Rural, Volume: 48, Issue: 11, Article number: e20180001, Published: 14 NOV 2018 |
| ISSN: | 1678-4596 |
| Popis: | EnglishThis study evaluated the effect of different concentrations of 2-isopentenyladenine (2-iP) on the direct somatic embryogenesis capacity of the Mundo Novo cultivar of Coffea arabica. Leaf explants were cultivated with half the MS salt concentration and the addition of sucrose (20gL-1) and 2-iP (0; 2.5; 5; 7.5 and 10µM). The 2-iP doses of 7.5 and 10µM produced the greatest responses with respect to the percentage of explants with embryogenic structures and the size of the embryogenic structures. However, the greatest production of somatic embryos occurred on the explants treated with 10µM of 2-iP, followed by 7.5µM, whereas their production was absent or reduced with 0 and 5µM, respectively. Key words: Cultivar Mundo Novo; 2-iP; somatic embryos; embryogenic structures portuguesEste estudo avaliou o efeito de diferentes concentracoes de 2-isopentalinadenina (2-iP) na capacidade de embriogenese somatica direta da cultivar Mundo Novo de Coffea arabica. Explantes foliares foram cultivados em meio com metade da concentracao de sais de MS e adicao de sacarose (20gL-1) e de 2-iP (0; 2,5; 5; 7,5 e 10µM). Os tratamentos com 7,5 e 10µM de 2-iP induziram respostas mais elevadas de porcentagem de explantes com estruturas embriogenicas e de tamanho de estrutura embriogenica. Porem, os explantes tratados 10µM de 2-iP tiveram maior producao de embrioes somaticos, seguido de 7,5µM, enquanto nos tratamentos controle e com 5µM, as respostas foram respectivamente de ausencia e de baixa formacao destes. Palavras chave: Cultivar Mundo Novo; 2-iP; embrioes somaticos; estrutura embriogenica |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|