Surface IgM on DT40 cells may be a component of the putative receptor complex responsible for the binding of infectious bursal disease virus
| Autor: | Gaiping Zhang, Man Teng, Jun Luo, Xue-Wu Li, Ming-lin Yi, Zhijun Xiao, Hong Zhang, Jian-Ming Fan, Leiming You, Yu-Bao Zhi |
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| Rok vydání: | 2010 |
| Předmět: |
Receptor complex
animal structures medicine.drug_class T7 phage Monoclonal antibody Infectious bursal disease virus Virus Infectious bursal disease Food Animals Peptide Library Cell Line Tumor medicine Animals Bursa of Fabricius Lymphocytes Infectivity General Immunology and Microbiology biology Membrane Proteins medicine.disease biology.organism_classification Virology Molecular biology Gene Expression Regulation Immunoglobulin M biology.protein Animal Science and Zoology Antibody Chickens Protein Binding |
| Zdroj: | Avian Pathology. 39:359-365 |
| ISSN: | 1465-3338 0307-9457 |
| DOI: | 10.1080/03079457.2010.506211 |
| Popis: | To investigate the host-pathogen interactions between infectious bursal disease virus (IBDV) and target B-lymphocytic cells, a cDNA T7 phage display library from the chicken bursa of Fabricius was constructed and screened for virus binding. Surface immunoglobulin M (sIgM) was isolated as a putative candidate binding site and its interactions with IBDV were further investigated using a chicken bursal lymphoma-derived cell line DT40. The results showed that the λ light chain of sIgM specifically interacted with IBDV in a virulence-independent manner in vitro, and most of the binding of IBDV to DT40 cells was inhibited by sIgM-specific monoclonal antibodies. Further, the infectivity of IBDV in vitro was reduced by sIgM-specific monoclonal antibodies. Our data provided evidence that sIgM may participate as one of the putative membrane binding sites responsible for IBDV infection. |
| Databáze: | OpenAIRE |
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