Structure elucidation of a bioactive fucomannogalactan from the edible mushroom Hypsizygus marmoreus
| Autor: | Sthefany Rodrigues Fernandes Viana, Luciano M. Lião, Stellee Marcela Petris Biscaia, E. S. Trindade, Maria Carolina Di Medeiros Leal, D. L. Bellan, Ana Flora Dalberto Vasconcelos, Ruberney S. Oliveira, Elaine R. Carbonero |
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| Přispěvatelé: | Universidade Federal de Goiás (UFG), Univ Fed Parana, Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Polymers and Plastics
B16-F10 cell Chemical structure Mannose Antineoplastic Agents 02 engineering and technology 010402 general chemistry Cell morphology Galactans 01 natural sciences Fucose Mushroom chemistry.chemical_compound Cell Movement Cell Line Tumor Materials Chemistry Animals Fruiting Bodies Fungal Molecular Structure Organic Chemistry Hypsizygus marmoreus 021001 nanoscience & nanotechnology In vitro 0104 chemical sciences Molecular Weight Edible mushroom Biochemistry chemistry Fucomannogalactan Galactose Antimelanoma Agaricales 0210 nano-technology |
| Zdroj: | Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2019-10-04T12:42:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-12-01 Made available in DSpace on 2019-10-04T12:42:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-12-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) A fucomannogalactan (FMG-Hm), with a molecular weight of 17.1 kDa, obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects. FMG-Hm was not cytotoxic, nor did it alter the cell morphology and proliferation, but was able to inhibit colony-forming ability and cell migration in B16-F10 murine melanoma cells. An analysis of the monosaccharide composition indicated that FMG-Hm was composed of fucose, mannose, and galactose in a ratio of 1.00:1.08:3.17. The FMG-Hm was structurally characterized based on methylation analysis, partial acid hydrolysis, and NMR experiments. The results indicated that FMG-Hm contained a alpha-(1 -> 6)-linked galactopyranosyl main chain, partially substituted at O-2 by non-reducing ends of alpha-L-fucopyranose and beta-D-mannopyranose. The predicted structure of the heteropolysaccharide was established as: [GRAPHICS] . Univ Fed Goias, Dept Quim, BR-75704020 Regional Catalao, Catalao, Brazil Univ Fed Parana, Dept Biol Celular, BR-81531980 Curitiba, Parana, Brazil Univ Estadual Paulista, Fac Ciencias Agron, Dept Engn Rural, BR-18610307 Botucatu, SP, Brazil Univ Fed Goias, Inst Quim, Lab Ressonancia Magnet Nucl, Campus Samambaia, BR-74001970 Goiania, Go, Brazil Univ Estadual Paulista, Fac Ciencias & Tecnol, Dept Quim & Bioquim, BR-19060900 Presidente Prudente, Brazil Univ Estadual Paulista, Fac Ciencias Agron, Dept Engn Rural, BR-18610307 Botucatu, SP, Brazil Univ Estadual Paulista, Fac Ciencias & Tecnol, Dept Quim & Bioquim, BR-19060900 Presidente Prudente, Brazil CAPES: 001 |
| Databáze: | OpenAIRE |
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