A panel of monoclonal antibodies recognizing the Staphylococcus epidermidis fibrinogen-binding MSCRAMM SdrG
| Autor: | Peter J. Syribeys, Paul Domanski, Andrea Hall, Elena Gorovits, Jeff T. Hutchins, Patel Pratisksha, John Vernachio, Bradley D. Prater, Joseph M. Patti |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.drug_class
Immunology Human pathogen Monoclonal antibody Virulence factor Microbiology Flow cytometry Mice Bacterial Proteins Staphylococcus epidermidis medicine Immunology and Allergy Animals Adhesins Bacterial Mice Inbred BALB C biology medicine.diagnostic_test Fibrinogen binding Antibodies Monoclonal Fibrinogen biology.organism_classification Virology Bacterial adhesin Lactococcus lactis Female MSCRAMM Carrier Proteins Protein Binding |
| Zdroj: | Hybridoma (2005). 26(1) |
| ISSN: | 1554-0014 |
| Popis: | Staphylococcus epidermidis is an important opportunistic human pathogen that has recently emerged as a major cause of foreign-body infections. The most important stage contributing to the pathogenesis of this bacteria is the initial adherence to host tissue. SdrG is a cell-wall–anchored fibrinogen-binding adhesin of S. epidermidis that has been shown to be necessary for bacterial binding to fibrinogen-coated foreign bodies, such as catheters. Here we report the generation and characterization of a panel of monoclonal antibodies (MAbs) directed against this S. epidermidis virulence factor. Through the use of multiple in vitro assays, surface plasmon resonance, and flow cytometry, we have characterized a diverse array of MAbs that may prove to be beneficial in studies that address the precise biologic role of SdrG. |
| Databáze: | OpenAIRE |
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