Anti-GD2-like IgM autoreactivity in multiple sclerosis patients
| Autor: | Silvia Marconi, Laura Lovato, Michele Acler, Bruno Bonetti, Elena Anghileri, L De Toni, Silvia Romito, C. Cordioli, Elisa Tedeschi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Population Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause Autoimmunity Pathogenesis Multiple sclerosis Disability Evaluation 03 medical and health sciences 0302 clinical medicine Autoantibodies Gangliosides GD2 Immunohistochemistry Myelin Antibody Specificity medicine Humans 030212 general & internal medicine education Myelin Sheath Aged education.field_of_study Expanded Disability Status Scale Autoantibody Middle Aged Multiple Sclerosis Chronic Progressive medicine.disease Oligodendroglia Immunoglobulin M Neurology Immunology Female lipids (amino acids peptides and proteins) Chromatography Thin Layer Neurology (clinical) 030217 neurology & neurosurgery Immunostaining |
| Popis: | Seric IgM autoreactivity in 100 multiple sclerosis (MS) and 106 control (70 of whom had other neurological diseases) patients was assessed either by immunohistochemistry on normal human CNS tissue or to GD2, GD1a, GD3 by ELISA and thin layer chromatography (TLC) techniques. By double immunohistochemistry, we found that 44% of the total MS population showed seric IgM reactivity to oligodendrocytes and myelin, this finding being particularly frequent in patients with secondary progressive MS. In the non-MS cohort, positive signals were seen only in one patient. In all cases, extraction of lipids from CNS sections abolished the immunoreactivity. Among the gangliosides investigated by ELISA, anti-GD2-like IgM autoantibodies were detected in the serum of 30% of MS patients, a subgroup of whom (below 10%) reacted also with GD1a and/or GD3. More than 85% of MS cases with anti-GD2-like IgM immunoreactivity by ELISA showed also IgM anti-oligodendrocyte/myelin staining by immunohistochemistry. However, no immunostaining in MS sera was observed when gangliosides were resolved by TLC. A positive correlation with neurological disability was observed, as the Expanded Disability Status Scale of MS patients with anti-GD2-like IgM autoreactivity by ELISA was significantly worse than seronegative MS cases. The results of the present study enforce the role of glycolipids as potential autoantigens and of IgM autoantibodies in MS pathogenesis. |
| Databáze: | OpenAIRE |
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