Internally Controlled, Multiplex Real-Time Reverse Transcription PCR for Dengue Virus and Yellow Fever Virus Detection
Autor: | Yvalena Guillén, Victoria Stittleburg, Ousmane Faye, Benjamin A. Pinsky, Amadou A. Sall, Alisha Mohamed-Hadley, Cheikh Tidiane Diagne, Oumar Faye, Angel Balmaseda, Jesse J. Waggoner, Alejandra Rojas, Eva Harris |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Serotype viruses 030106 microbiology Dengue virus Biology medicine.disease_cause Virus Dengue fever Dengue 03 medical and health sciences Virology Multiplex polymerase chain reaction Yellow Fever medicine Humans Multiplex Reverse Transcriptase Polymerase Chain Reaction Yellow fever virus diseases Articles biochemical phenomena metabolism and nutrition Dengue Virus medicine.disease Reverse transcription polymerase chain reaction 030104 developmental biology Infectious Diseases Early Diagnosis Parasitology Yellow fever virus Multiplex Polymerase Chain Reaction |
Popis: | The differential diagnosis of dengue virus (DENV) and yellow fever virus (YFV) infections in endemic areas is complicated by nonspecific early clinical manifestations. In this study, we describe an internally controlled, multiplex real-time reverse transcription polymerase chain reaction (rRT-PCR) for the detection of DENV and YFV. The DENV–YFV assay demonstrated specific detection and had a dynamic range of 2.0–8.0 log(10) copies/μL of eluate for each DENV serotype and YFV. Clinical performance was similar to a published pan-DENV assay: 48/48 acute-phase samples from dengue cases were detected in both assays. For YFV detection, mock samples were prepared with nine geographically diverse YFV isolates over a range of concentrations. The DENV–YFV assay detected 62/65 replicates, whereas 54/65 were detected using a reference YFV rRT-PCR. Given the reemergence of DENV and YFV in areas around the world, the DENV–YFV assay should be a useful tool to narrow the differential diagnosis and provide early case detection. |
Databáze: | OpenAIRE |
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