Determination of meropenem in serum by high-performance liquid chromatography with column switching
| Autor: | L. Ferrante, M. De Martinis, S. Bompadre, L. Leone |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Detection limit
Chromatography Chemistry Organic Chemistry Extraction (chemistry) Analytical chemistry General Medicine Reversed-phase chromatography Biochemistry High-performance liquid chromatography Analytical Chemistry Blood serum meropenem Solvents Humans chromatography Indicators and Reagents Spectrophotometry Ultraviolet Thienamycins pharmacokinetics Sample preparation Solid phase extraction Chromatography High Pressure Liquid Antibacterial agent |
| Zdroj: | Journal of Chromatography A. 812:249-253 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(98)00249-0 |
| Popis: | A rapid, accurate and sensitive liquid chromatographic assay with on-line solid-phase extraction for determination of meropenem in serum is described. Sample was directly injected onto the extraction column for sample clean-up and extraction. Thereafter, using an on-line column-switching system the drug was quantitatively transferred and separated on a C18 analytical column. Ultraviolet absorption at 298 nm was used for detection. The assay was linear from 1 to 100 micrograms/ml. Recovery was 98.5%. Based on a 20-microliters sample volume (serum- water, 1:1, v/v), detection limit was 0.1 microgram/ml. An application of the method to study the pharmacokinetics of meropenem is given. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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