Proteogenomic analysis of Georgfuchsia toluolica revealed unexpected concurrent aerobic and anaerobic toluene degradation

Autor: Peng Peng, Mike S. M. Jetten, Alfons J. M. Stams, Jeroen Frank, Margreet J. Oosterkamp, Hauke Smidt, David Schleheck, Siavash Atashgahi, Bastian V. H. Hornung, Beate Kraft, Sebastian Lücker
Přispěvatelé: Universidade do Minho
Rok vydání: 2021
Předmět:
Zdroj: Atashgahi, S, Oosterkamp, M, Peng, P, Frank, J, Kraft, B, Hornung, B, Schleheck, D, Lücker, S, Jetten, M, Stams, A & Smidt, H 2021, ' Proteogenomic analysis of Georgfuchsia toluolica revealed unexpected concurrent aerobic and anaerobic toluene degradation ', Environmental Microbiology Reports, vol. 13, no. 6, pp. 841-851 . https://doi.org/10.1111/1758-2229.12996
Environmental Microbiology Reports, 13(6), 841-851
Environmental Microbiology Reports, 13, 841-851
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
Environmental Microbiology Reports 13 (2021) 6
Environmental Microbiology Reports, 13, 6, pp. 841-851
ISSN: 1758-2229
Popis: Denitrifying Betaproteobacteria play a key role in the anaerobic degradation of monoaromatic hydrocarbons. We performed a multi-omics study to better understand the metabolism of the representative organism Georgfuchsia toluolica strain G5G6 known as a strict anaerobe coupling toluene oxidation with dissimilatory nitrate and Fe(III) reduction. Despite the genomic potential for degradation of different carbon sources, we did not find sugar or organic acid transporters, in line with the inability of strain G5G6 to use these substrates. Using a proteomics analysis, we detected proteins of fumarate-dependent toluene activation, membrane-bound nitrate reductase, and key components of the metal-reducing (Mtr) pathway under both nitrate- and Fe(III)-reducing conditions. High abundance of the multiheme cytochrome MtrC implied that a porincytochrome complex was used for respiratory Fe(III) reduction. Remarkably, strain G5G6 contains a full set of genes for aerobic toluene degradation, and we detected enzymes of aerobic toluene degradation under both nitrate- and Fe(III)-reducing conditions. We further detected an ATP-dependent benzoyl-CoA reductase, reactive oxygen species detoxification proteins, and cytochrome c oxidase indicating a facultative anaerobic lifestyle of strain G5G6. Correspondingly, we found diffusion through the septa a substantial source of oxygen in the cultures enabling concurrent aerobic and anaerobic toluene degradation by strain G5G6.
This work was supported by Wageningen University & Research through its investment theme Resilience, the Technology Foundation (STW), the Applied Science Division of the Dutch Research Council (NWO; project 08053), NWO grant 016.Vidi.189.050, and a Gravitation grant of the Netherlands Ministry of Education, Culture and Science and NWO (project 024.002.002 SIAM). B.K. was supported by the Villum foundation, Denmark (VYI Grant 25491).
info:eu-repo/semantics/publishedVersion
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje