The use of polyethylenimine–DNA to topically deliver hTERT to promote hair growth
| Autor: | Lin Sj, Lai Ps, Peng Cl, Wei Mf, Jan Hm, Ming-Jium Shieh |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Keratinocytes
Time Factors Cell Survival Transfection Neogenesis Flow cytometry Mice Follicle Proliferating Cell Nuclear Antigen Genetics medicine Animals Humans Polyethyleneimine Regeneration Telomerase reverse transcriptase Rats Wistar Telomerase Molecular Biology Cell Proliferation Swiss 3T3 Cells integumentary system biology medicine.diagnostic_test Cell growth Stem Cells Gene Transfer Techniques DNA Genetic Therapy Flow Cytometry beta-Galactosidase biology.organism_classification Molecular biology In vitro Rats Proliferating cell nuclear antigen embryonic structures biology.protein Wounds and Injuries Molecular Medicine Hair Follicle Plasmids |
| Zdroj: | Gene Therapy. 19:86-93 |
| ISSN: | 1476-5462 0969-7128 |
| Popis: | The present study investigates the efficacy of polyethylenimine (PEI)-DNA complex that expressed human telomerase reverse transcriptase (hTERT) to transfect hair follicle stem cells and produce sufficient hTERT to stimulate hair growth. Transfection with pLC-hTERT-DNA-PEI complex (D+P group) in vitro induced expression of proliferating cell nuclear antigen in 35.8% of the purified stem cell population, suggesting enhanced cell proliferation. In vivo transfection efficiency of rat dorsal skin was determined by staining for β-gal activity. Cells positive for β-gal were located in the bulge region and dermal sheath of hair follicles. The follicles in the hTERT-transfected region entered anagenon day 15 after transfection, whereas non-transfected (Neg) controls remained in telogen. The similar effect was observed in 50-day-old rat dorsal skin. D+P group displayed a specific expression of hTERT and sufficient to initiate a transition to the anagen phase and promote new hair synthesis 18 days after the transfection. hTERT promoted follicle neogenesis following wounding. In all, 60 days after wounding, tissues of the D+P group showed more newly regenerating hair follicles (83±52 regenerated follicles per rat) in contrast to control group tissues (15±15 regenerated follicles per rat). These studies provide a potential approach for gene therapy of skin disease. |
| Databáze: | OpenAIRE |
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