Pehuén (Araucaria araucana) seed residues are a valuable source of natural antioxidants with nutraceutical, chemoprotective and metal corrosion-inhibiting properties

Autor: Ana Ferrari, Ignasi Queralt, María C. Gallia, Marcelo Adrián Mazzeo, Guillermina A. Bongiovanni, Evelin Bachmeier
Přispěvatelé: Ministerio de Ciencia e Innovación (España)
Rok vydání: 2020
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
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ISSN: 1090-2120
Popis: In the last decade, green chemistry has been attracting great interest in many contexts, including, among others, natural antioxidants. However, only a few works deal with natural residue extracts and biowaste, which could be an efficient, economical and environmentally friendly source for the production of useful compounds. In this study, we look for antioxidant activity in Araucaria araucana seeds, an iconic pine species of the Argentine and Chilean Patagonia commonly known as “pehuén”. Piñones are the edible pehuén seeds, and it is estimated that approximately 40 tons of piñones are harvested annually in Argentina and Chile. The chemical composition, antioxidants, metal corrosion-inhibiting properties and biological activity of edible and discarded piñón tissues were determined. Acute toxicity was discarded by in vitro testing and double fluorescent staining. Biological activity was evaluated in vivo by determining redox markers in salivary glands from rats treated with Cyclophosphamide (an oncological drug). All piñón tissues had antioxidants and antioxidant activity, with the coats showing the highest levels (up to 404 µg ascorbic acid equivalent per mg). The coats, in particular, had high gallic acid, catechin, quercetin and tannin contents, and more antioxidant activity, polyphenols and flavonoids than berries from the region. Results by X-ray fluorescence spectrometry showed that Na, Mg, P, S, Cl, K, and Ca were majority elements in the coat, embryo and endosperm. Furthermore, coat extract also showed significant anti-corrosion activity and in vivo protection against oxidative damage. The results indicate that piñón biowaste is a low-cost attractive source of natural antioxidants with potential nutraceutical, medical and metal corrosion protection applications.
This work was supported by the FONCyT (PICT 2013-0458-2014/2018), the CONICET (PIP0239-2016/2019, PUE0067-2017-2021), the National University of Comahue (PIN04/N025-2015/2019), and the Ministry of Education of Argentina (VT12-UNCOMA4094-2016/2017), SeCyT-UNC (33620180100930CB). Elemental analysis work was developed in the framework of activities of the CEX2018-000794-S project (Spanish Ministry of Science and Innovation). The authors thank Nelda B. Gaydou, CT, American Translators Association Member No 5328, for proofreading the manuscript, and Ing. René Valero, Dra. Carmen Maturano, Bioq. Jorge A. Linares, Od. María M. López, Ing. Leonardo Bajda and Qca. Marcela Amaro for their technical assistance during sample preparation and HPLC determinations. The authors also appreciate the kind collaboration of Yanina and Norma from NOUS in Villa Pehuenia, Neuquén, the Villa Pehuenia municipal authorities, and the AIC (Interjurisdictional Authority of the Limay, Neuquén and Negro River Basins) for the meteorological data provided.
Databáze: OpenAIRE
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