Induction of Apoptosis in Human Lung Carcinoma A549 Epithelial Cells with an Ethanol Extract ofTremella mesenterica
| Autor: | Tai-Hao Hsu, Fang-Yi Lin, Nan-Yin Chen, Hsi-Huai Lai, Hui-Chen Lo, Jian-Zhi Chen |
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| Rok vydání: | 2008 |
| Předmět: |
Cell Extracts
Lung Neoplasms Cell Survival Apoptosis Applied Microbiology and Biotechnology Biochemistry Analytical Chemistry Polysaccharides Annexin Cell Line Tumor Humans Viability assay Molecular Biology Membrane Potential Mitochondrial chemistry.chemical_classification A549 cell Reactive oxygen species Ethanol biology Caspase 3 Basidiomycota Organic Chemistry Epithelial Cells General Medicine biology.organism_classification Molecular biology Enzyme Activation chemistry Cell culture Extracellular Space Reactive Oxygen Species Tremella mesenterica Intracellular Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 72:1283-1289 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.70773 |
| Popis: | Tremella mesenterica (TM) is a common food and folk medicine widely used in several Asian countries as a tonic for the lungs. In the present study, we compared the effects of extracellular polysaccharides (EPS), intracellular polysaccharides (IPS), and ethanol extract (EE) of Tremella mesenterica on the induction of apoptosis into human lung carcinoma A549 epithelial cells. The EE, but not the EPS or the IPS, almost completely inhibited the growth of A549 cells. The results of Annexin V-FITC/PI staining and flow cytometric analysis indicated that the percentage of Annexin V(+)/PI(-) cells in EE-treated cells increased to 32.8%. The results of further investigation showed a disruption of mitochondrial transmembrane potential (DeltaPsi(m)), the production of reactive oxygen species (ROS), and the activation of caspase-3 protein in EE-treated cells. These findings suggest that EE can decrease cell viability and induce apoptosis in A549 cell lines by activating a mitochondrial pathway. |
| Databáze: | OpenAIRE |
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