A Non-Enzyme and Non-Label Sensitive Fluorescent Aptasensor Based on Simulation-Assisted and Target-Triggered Hairpin Probe Self-Assembly for Ochratoxin a Detection
| Autor: | Wenxiao Hu, Yafei Dong, Yue Wang, Luhui Wang, Mengyao Qian |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Ochratoxin A
computation Health Toxicology and Mutagenesis Aptamer lcsh:Medicine Biosensing Techniques 02 engineering and technology Toxicology G-quadruplex biosensor 01 natural sciences Article chemistry.chemical_compound Fluorometry Fluorescent Dyes chemistry.chemical_classification lcsh:R 010401 analytical chemistry Aptamers Nucleotide 021001 nanoscience & nanotechnology simulation Ochratoxins Fluorescence Combinatorial chemistry 0104 chemical sciences G-Quadruplexes Enzyme Mesoporphyrins chemistry Nucleic acid Self-assembly fluorescence 0210 nano-technology Biosensor ochratoxin A |
| Zdroj: | Toxins Volume 12 Issue 6 Toxins, Vol 12, Iss 376, p 376 (2020) |
| ISSN: | 2072-6651 |
| DOI: | 10.3390/toxins12060376 |
| Popis: | The monitoring and control of mycotoxins has caused widespread concern due to their adverse effects on human health. In this research, a simple, sensitive and non-label fluorescent aptasensor has been reported for mycotoxin ochratoxin A (OTA) detection based on high selectivity of aptamers and amplification of non-enzyme hybridization chain reaction (HCR). After the introduction of OTA, the aptamer portion of hairpin probe H1 will combine with OTA to form OTA-aptamer complexes. Subsequently, the remainder of the opened H1 will act as an initiator for the HCR between the two hairpin probes, causing H1 and H2 to be sequentially opened and assembled into continuous DNA duplexes embedded with numerous G-quadruplexes, leading to a significant enhancement in fluorescence signal after binding with N-methyl-mesoporphyrin IX (NMM). The proposed sensing strategy can detect OTA with concentration as low as 4.9 pM. Besides, satisfactory results have also been obtained in the tests of actual samples. More importantly, the thermodynamic properties of nucleic acid chains in the monitoring platform were analyzed and the reaction processes and conditions were simulated before carrying out biological experiments, which theoretically proved the feasibility and simplified subsequent experimental operations. Therefore, the proposed method possess a certain application value in terms of monitoring mycotoxins in food samples and improving the quality control of food security. |
| Databáze: | OpenAIRE |
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