Oncopeltus-like gene expression patterns in Murgantia histrionica, a new hemipteran model system, suggest ancient regulatory network divergence
| Autor: | Jessica Hernandez, Leslie Pick, Katie Reding |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Harlequin bug
media_common.quotation_subject Pair-rule gene lcsh:Evolution Insect Hemiptera Segmentation Pentatomidae Genetics lcsh:QH359-425 Drosophila Ecology Evolution Behavior and Systematics media_common Murgantia histrionica biology Research Runt fungi biology.organism_classification Insect model system Evolutionary biology E75A Pair-rule genes Drosophila melanogaster Developmental biology Developmental Biology |
| Zdroj: | EvoDevo, Vol 11, Iss 1, Pp 1-14 (2020) EvoDevo |
| DOI: | 10.13016/ppyk-ucmp |
| Popis: | BackgroundMuch has been learned about basic biology from studies of insect model systems. The pre-eminent insect model system,Drosophila melanogaster, is a holometabolous insect with a derived mode of segment formation. While additional insect models have been pioneered in recent years, most of these fall within holometabolous lineages. In contrast, hemimetabolous insects have garnered less attention, although they include agricultural pests, vectors of human disease, and present numerous evolutionary novelties in form and function. The milkweed bug,Oncopeltus fasciatus(order: Hemiptera)—close outgroup to holometabolous insects—is an emerging model system. However, comparative studies within this order are limited as many phytophagous hemipterans are difficult to stably maintain in the lab due to their reliance on fresh plants, deposition of eggs within plant material, and long development time from embryo to adult.ResultsHere we present the harlequin bug,Murgantia histrionica, as a new hemipteran model species.Murgantia—a member of the stink bug family Pentatomidae which shares a common ancestor withOncopeltus ~ 200 mya—is easy to rear in the lab, produces a large number of eggs, and is amenable to molecular genetic techniques. We useMurgantiato ask whether Pair-Rule Genes (PRGs) are deployed in ways similar to holometabolous insects or toOncopeltus. Specifically, PRGseven-skipped, odd-skipped, pairedandsloppy-pairedare initially expressed in PR-stripes inDrosophilaand a number of holometabolous insects but in segmental-stripes inOncopeltus. We found that these genes are likewise expressed in segmental-stripes inMurgantia,whileruntdisplays partial PR-character in both species. Also likeOncopeltus,E75Ais expressed in a clear PR-pattern in blastoderm- and germband-stageMurgantiaembryos, although it plays no role in segmentation inDrosophila. Thus, genes diagnostic of the split between holometabolous insects andOncopeltusare expressed in anOncopeltus-like fashion duringMurgantiadevelopment.ConclusionsThe similarity in gene expression betweenMurgantiaandOncopeltussuggests thatOncopeltusis not a sole outlier species in failing to utilize orthologs ofDrosophilaPRGs for PR-patterning. Rather, strategies deployed for PR-patterning, including the use ofE75Ain the PRG-network, are likely conserved within Hemiptera, and possibly more broadly among hemimetabolous insects. |
| Databáze: | OpenAIRE |
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