Carrying-over effects of GVBD blocking on post-blocking meiotic progression of oocytes: species difference and the signaling pathway leading to MPF activation
| Autor: | Yan Gao, Liang-Liang Zheng, Guang-Zhong Jiao, Chuan-Xin Zhang, Jing-He Tan, Hua-Yu Lian, Ming-Ju Sun, Shuai Gong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Maturation-Promoting Factor
Sus scrofa Cyclin B lcsh:Medicine Cell Cycle Proteins Biology Cycloheximide chemistry.chemical_compound Mice Species Specificity Epidermal growth factor Cyclins Roscovitine Animals Cell Cycle and Cell Division Protein Precursors lcsh:Science Cell Cycle Inhibitors Cells Cultured Cyclin Multidisciplinary Germinal vesicle urogenital system lcsh:R Biology and Life Sciences Cell Biology In vitro Cell biology Meiosis chemistry Cell Processes Purines Mesothelin biology.protein Oocytes Phosphorylation Female lcsh:Q Signal transduction Research Article Meiotic Prophase Signal Transduction |
| Zdroj: | PLoS ONE, Vol 9, Iss 7, p e103838 (2014) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Efforts to improve the quality of in vitro matured oocytes by blocking germinal vesicle breakdown (GVBD) and allowing more time for ooplasmic maturation have achieved little due to a lack of knowledge on the molecular events during GVBD blocking. Such knowledge is also important for studies aimed at regulating gene expression in maturing oocytes prior to GVBD. We studied species difference and signaling pathways leading to the carrying-over effect of GVBD blocking on post-blocking meiotic progression (PBMP). Overall, GVBD-blocking with roscovitine decelerated PBMP of mouse oocytes but accelerated that of pig oocytes. During blocking culture, whereas cyclin B of pig oocytes increased continuously, that of mouse oocytes declined first and then increased slowly. In both species, (a) whereas active CDC2A showed a dynamics similar to cyclin B, inactive CDC2A decreased continuously; (b) when oocytes were blocked in blocking medium containing cycloheximide, PBMP was decelerated significantly while cyclin B and active CDC2A decreasing to the lowest level; (c) whereas sodium vanadate in blocking medium reduced PBMP, epidermal growth factor (EGF) in blocking medium accelerated PBMP significantly with no effect on cyclin B levels. In conclusion, the EGF signaling cascade accelerated PBMP by promoting the pre-MPF (M-phase-promoting factor) to MPF conversion during GVBD blocking with roscovitine. The significant difference in PBMP observed between mouse and pig oocytes was caused by species difference in cyclin B dynamics during blocking culture as no species difference was observed in either pre-MPF to MPF conversion or the EGF signaling activity. |
| Databáze: | OpenAIRE |
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